Evaluation of DNA barcoding coupled high resolution melting for discrimination of closely related species in phytopharmaceuticals
文献类型：期刊论文
| 作者 | Osathanunkul M[*]1; Suwannapoom C2,3; Osathanunkul K4; Madesis P5; de Boer H6,7 |
| 刊名 | PHYTOMEDICINE
 |
| 出版日期 | 2016 |
| 卷号 | 23期号:2页码:156-65. |
| 关键词 | Authentication DNA barcoding Herbal pharmacovigilance High resolution melting Medicinal plants |
| 通讯作者 | omaslin@gmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Background: Phytopharmaceuticals are increasingly popular as alternative medicines, but poorly regulated in many countries. The manufacturers of these products should be subject to strict controls regarding each product's quality and constituents. Routine testing and identification of raw materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. Hypothesis/purpose: We have applied DNA Barcoding High Resolution Melting (Bar-HRM), an emerging method for identifying of medicinal plant species based on DNA dissociation kinetics and DNA barcoding, for the authentication of medicinal plant species. Study design: Commonly commercialized Thai medicinal plants that are widely used for medicinal purposes were used in this study. Publicly available sequences of four plastid markers were used for universal primer design. Species discrimination efficiency of the designed primers was evaluated as single and multi-locus analyses by using the primers sets. Methods: HRM analysis was performed in triplicate on each of the 26 taxa to establish the T-m for each primer set (matK, rbcLA, rbcLB, rbcLC, rpoC1, and trnL). The shapes of the melting curves were analyzed to distinguish the different plant species. Bar-HRM species identification success rates were assessed for each single-locus as well as for multi-locus combinations to establish the optimal combination of primer sets. Results: In single locus analysis the rpoC1 primer set gave the highest discrimination (53%), and in multi locus analysis this could be increased from 87% to 99% depending on the total number of regions included. Different combinations proved to be more or less effective at discrimination, depending on the genus or family examined. Conclusions: Bar-HRM has proven to be a cost-effective and reliable method for the identification of species in this study of Thai medicinal plants, and results show an identification success rate of 99% among species in the test set. |
| 收录类别 | SCI |
| 资助信息 | This research was financially supported by Thailand Research Fund New Researcher Grant TRG5780027 awarded to MO. HdB ac- knowledges support from the Norway–Romania EEA Research Pro- gramme operated by the MECS-ANCSI PO under the EEA Financial Mechanism 2009–2014 Project Contract no. 2SEE/2014. We thank late Mr. James F. Maxwell (Curator of CMUB herbarium, Chiang Mai University) and Mr. Samphan Wongthep (Medicinal Plant Garden, Chiang Mai University) for providing, identifying and collecting the samples. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9569]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Department of Biology, Faculty of Science, Chiang Mai University, 239 Huay Kaew Rd., Suthep, Muang, Chiang Mai 50200, Thailand 2.State Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand 4.Department of Computer Science, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand 5.Institute of Applied Biosciences, Centre for Research & Technology Hellas (CERTH), Thessaloniki, Greece 6.Department of Organismal Biology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, SE-75236 Uppsala, Sweden 7.The Natural History Museum, University of Oslo, P.O. Box 1172, NO-0318 Oslo, Norway |
| 推荐引用方式 GB/T 7714 | Osathanunkul M[*],Suwannapoom C,Osathanunkul K,et al. Evaluation of DNA barcoding coupled high resolution melting for discrimination of closely related species in phytopharmaceuticals[J]. PHYTOMEDICINE,2016,23(2):156-65.. |
| APA | Osathanunkul M[*],Suwannapoom C,Osathanunkul K,Madesis P,&de Boer H.(2016).Evaluation of DNA barcoding coupled high resolution melting for discrimination of closely related species in phytopharmaceuticals.PHYTOMEDICINE,23(2),156-65.. |
| MLA | Osathanunkul M[*],et al."Evaluation of DNA barcoding coupled high resolution melting for discrimination of closely related species in phytopharmaceuticals".PHYTOMEDICINE 23.2(2016):156-65.. |
入库方式： OAI收割
来源：昆明动物研究所
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