Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma
文献类型：期刊论文
| 作者 | Xu XP1; Chen HB1; Cao XM1,2; Ben KL[*]1,2 |
| 刊名 | JOURNAL OF GENERAL VIROLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 88期号:X页码:2504-2512 子辑: 9 |
| 通讯作者 | kben@vip.sina.com |
| 英文摘要 | The generation of a new, cost-effective, non-primate, small-animal model would greatly facilitate research into hepatitis C virus (HCV) pathogenesis and the development of novel therapeutic and preventative technologies to control the increasing HCV threat to public health. Native HCV from patient plasma and HCV grown in cell culture (HCVcc) were used to inoculate adult tree shrews. Each animal was inoculated with one HCV genotype. Alanine aminotransferase (ALT) levels, HCV RNA and viral load were determined in the animals before and after inoculation. For native HCV, 16/18 inoculated tree shrews (89 %) became infected; 12/16 (75 %) of these animals became chronically infected, whilst infection was resolved in the remaining four (25%). For HCVcc, infection occurred in 10/12 inoculated tree shrews (83 %) and chronic infection was observed in two of these animals. HCVcc from Huh7 cells showed a higher infectivity than that from HeLa cells. The animals inoculated with inadequate amounts of HCV were not infected in either native HCV or HCVcc experiments. Peak viral loads reached 10(3) -10(5) international units ml(-1) in chronically infected animals. ALT level changes reflected the normal fluctuation range in most animals. Thus, tree shrews without immunosuppression can be infected efficiently by native HCV and HCVcc when the animal is inoculated with an adequate amount of single-genotype HCV. |
| 收录类别 | SCI |
| 资助信息 | We are grateful to Mr Zhenqiu Chen for providing concentrated HCVcc, Mrs Xueshan Xia and Wenhua Zhao for assistance in HCV genotyping, Drs Yanwei Qi and Junxin Zhang for patient consulta- tion, Mrs Yunzhen Wei for animal maintenance and care, and Mr Weiyun Li for partial support from the China 863 Program. This work was supported in part by the Science Innovation Program of the Chinese Academy of Sciences (1999–2004) and China 863 Program (no. 2002AA216031). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9633]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.Laboratory for Molecular and Cellular Immunology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Kunming Chinawave Biotechnology Co. Ltd, Kunming, Yunnan 650106, China |
| 推荐引用方式 GB/T 7714 | Xu XP,Chen HB,Cao XM,et al. Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma[J]. JOURNAL OF GENERAL VIROLOGY,2007,88(X):2504-2512 子辑: 9. |
| APA | Xu XP,Chen HB,Cao XM,&Ben KL[*].(2007).Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma.JOURNAL OF GENERAL VIROLOGY,88(X),2504-2512 子辑: 9. |
| MLA | Xu XP,et al."Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma".JOURNAL OF GENERAL VIROLOGY 88.X(2007):2504-2512 子辑: 9. |
入库方式： OAI收割
来源：昆明动物研究所
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