COI-based barcoding of Chinese vipers (Reptilia: Squamata: Viperidae)
文献类型：期刊论文
| 作者 | Liu Q1,2; Zhu F1,2; Xiao R1; Cai YS5; Guo P[*]1; Zhong GH1,3; Wang YY4; Fang M1 |
| 刊名 | AMPHIBIA-REPTILIA
 |
| 出版日期 | 2015 |
| 卷号 | 36期号:4页码:361-372 |
| 关键词 | Asia snake species identification Viperidae |
| 通讯作者 | ybguop@163.com |
| 英文摘要 | DNA barcoding seeks to assemble a standardized reference library for rapid and unambiguous identification of species, and can be used to screen for potentially cryptic species. The 5' region of cytochrome oxidase subunit I (COI), which is a mitochondrial DNA (mtDNA) gene fragment, has been proposed as a universal marker for this purpose among animals. However, DNA barcoding of reptiles is still supported only by few datasets compared with other groups. We investigated the utilization of COI to discriminate 34 putative species of vipers, representing almost 92% of the recorded species in China. Based on a total of 241 sequences, our results indicated that the average degree of intraspecific variability (0.0198) tends to be one-sixth the average of interspecific divergence (0.0931), but no barcoding gap was detected between them. The threshold method, BLOG analyses and tree-based methods all can identify species with a high success rate. These results consistently suggested the usefulness and reliability of the DNA barcoding approach in Chinese vipers. |
| 收录类别 | SCI |
| 资助信息 | This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 31071892, NSFC 31372152, NSFC 31090251), Ministry of Science and Technology of the People’s Republic of China (2011FY120200), the Department of education of Sichuan Province (13TD0027), the Animal Branch of the Germplasm Bank of Wild Species, the Chinese Academy of Sciences (the Large Research infrastructure Funding). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10345]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.College of Life Sciences and Food Engineering, Yibin University, Yibin 644007, China 2.College of Life Sciences, Sichuan University, Chengdu 610064, China 3.CollegeofTourismandUrban-RuralPlanning,Chengdu University of Technology, Chengdu 610059, China 4.State Key Laboratory of Genetic Resources and Evolu- tion, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China 5.Department of Medical Biology and Genetics, Luzhou Medical College, Luzhou, 646000, China |
| 推荐引用方式 GB/T 7714 | Liu Q,Zhu F,Xiao R,et al. COI-based barcoding of Chinese vipers (Reptilia: Squamata: Viperidae)[J]. AMPHIBIA-REPTILIA,2015,36(4):361-372. |
| APA | Liu Q.,Zhu F.,Xiao R.,Cai YS.,Guo P[*].,...&Fang M.(2015).COI-based barcoding of Chinese vipers (Reptilia: Squamata: Viperidae).AMPHIBIA-REPTILIA,36(4),361-372. |
| MLA | Liu Q,et al."COI-based barcoding of Chinese vipers (Reptilia: Squamata: Viperidae)".AMPHIBIA-REPTILIA 36.4(2015):361-372. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。