Aniracetam restores the effects of amyloid-beta protein or ageing on membrane fluidity and intracellular calcium concentration in mice synaptosomes
文献类型：期刊论文
| 作者 | Li Y1,2,3; Wang JJ3; Cai JX[*]2 |
| 刊名 | JOURNAL OF NEURAL TRANSMISSION
 |
| 出版日期 | 2007 |
| 卷号 | 114期号:11页码:1407-1411 |
| 关键词 | aniracetam membrane fluidity amyloid-beta protein 25-35 synaptosome calcium mice |
| 通讯作者 | Caijx@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | In the present study, we observed the in vitro effect of aniracetam on membrane fluidity and free calcium concentrations (Ca(2+)i) of frontal cortical (FC) and hippocampal (HP) synaptosomes of aged mice and young mice treated with amyloid-beta protein (A beta) Membrane fluidity was measured by using fluorescence anisotropy of the lipophilic probe, 1,6-diphenyl-1,3,5-hexatriene (DPH). Ca(2+)i was measured by using Fura 2-AM fluorescent spectrophotometry. We found that membrane fluidity of the FC and HP synaptosomes was decreased in 14 months old mice compared with that in 3 months old mice. Similarly, A beta 25-35 (1 +/-mu M) decreased the membrane fluidity in 3 months old mice. These effects of ageing and A beta 25-35 on membrane fluidity were restored by aniracetam in a concentration-dependent manner. Furthermore, A beta 25-35 (1 +/-mu M) largely increased Ca2+ in FC and HP synaptosomes in 3 months old mice, but this effect on HP synaptosomes was effectively reversed by aniracetam (1-4 +/- mM). The present findings suggest that aniracetam restores age- and A beta-induced alterations in membrane fluidity or A beta-induced increase in Ca2+, demonstrating a possible beneficial role of aniracetam in the clinic treatment for senile dementia or Alzheimer's disease. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by National Natural Sciences Fund of China 30070251 and 39830440, and Yunnan Province Sciences Fund 2000C0006Z, and the Scientific Research Foundation of Qufu Normal University. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10520]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.College of Life Sciences, Qufu Normal University, Qufu, P.R. China 2.Chinese Academy of Sciences, Section of Brain and Behavior, Kunming Institute of Zoology, Kunming, P.R. China 3.Department of Biological Science and Technology and the State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, P.R. China |
| 推荐引用方式 GB/T 7714 | Li Y,Wang JJ,Cai JX[*]. Aniracetam restores the effects of amyloid-beta protein or ageing on membrane fluidity and intracellular calcium concentration in mice synaptosomes[J]. JOURNAL OF NEURAL TRANSMISSION,2007,114(11):1407-1411. |
| APA | Li Y,Wang JJ,&Cai JX[*].(2007).Aniracetam restores the effects of amyloid-beta protein or ageing on membrane fluidity and intracellular calcium concentration in mice synaptosomes.JOURNAL OF NEURAL TRANSMISSION,114(11),1407-1411. |
| MLA | Li Y,et al."Aniracetam restores the effects of amyloid-beta protein or ageing on membrane fluidity and intracellular calcium concentration in mice synaptosomes".JOURNAL OF NEURAL TRANSMISSION 114.11(2007):1407-1411. |
入库方式： OAI收割
来源：昆明动物研究所
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