Environmental DNA from Residual Saliva for Efficient Noninvasive Genetic Monitoring of Brown Bears (Ursus arctos)
文献类型：期刊论文
| 作者 | Wheat RE[*]1; Allen JM2; Miller SD3,4; Wilmers CC1; Levi T2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2016 |
| 卷号 | 11期号:11页码:e0165259 |
| 通讯作者 | rwheat@ucsc.edu |
| 合作状况 | 其它 |
| 英文摘要 | Noninvasive genetic sampling is an important tool in wildlife ecology and management, typically relying on hair snaring or scat sampling techniques, but hair snaring is labor and cost intensive, and scats yield relatively low quality DNA. New approaches utilizing environmental DNA (eDNA) may provide supplementary, cost-effective tools for noninvasive genetic sampling. We tested whether eDNA from residual saliva on partially-consumed Pacific salmon (Oncorhynchus spp.) carcasses might yield suitable DNA quality for noninvasive monitoring of brown bears (Ursus arctos). We compared the efficiency of monitoring brown bear populations using both fecal DNA and salivary eDNA collected from partially-consumed salmon carcasses in Southeast Alaska. We swabbed a range of tissue types from 156 partially-consumed salmon carcasses from a midseason run of lakeshore-spawning sockeye (O. nerka) and a late season run of stream-spawning chum (O. keta) salmon in 2014. We also swabbed a total of 272 scats from the same locations. Saliva swabs collected from the braincases of salmon had the best amplification rate, followed by swabs taken from individual bite holes. Saliva collected from salmon carcasses identified unique individuals more quickly and required much less labor to locate than scat samples. Salmon carcass swabbing is a promising method to aid in efficient and affordable monitoring of bear populations, and suggests that the swabbing of food remains or consumed baits from other animals may be an additional cost-effective and valuable tool in the study of the ecology and population biology of many elusive and/or wide-ranging species. |
| 收录类别 | SCI |
| 资助信息 | This work was funded by an NSFGRF to REW,and grants from the International Bear Association,the Alaska Chilkoot Bear Foundation, and the National Geographic Society(grant#14- 1544).Publication of this paper was supported ,in part,by the Thomas G.Scott Publication Fund. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10645]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Department of Environmental Studies, Center for Integrated Spatial Research, University of California Santa Cruz, Santa Cruz, California, United States of Americ 2.Department of Fisheries and Wildlife, Oregon State University, Corvallis, Oregon, United States of America 3.Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming, China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China |
| 推荐引用方式 GB/T 7714 | Wheat RE[*],Allen JM,Miller SD,et al. Environmental DNA from Residual Saliva for Efficient Noninvasive Genetic Monitoring of Brown Bears (Ursus arctos)[J]. PLOS ONE,2016,11(11):e0165259. |
| APA | Wheat RE[*],Allen JM,Miller SD,Wilmers CC,&Levi T.(2016).Environmental DNA from Residual Saliva for Efficient Noninvasive Genetic Monitoring of Brown Bears (Ursus arctos).PLOS ONE,11(11),e0165259. |
| MLA | Wheat RE[*],et al."Environmental DNA from Residual Saliva for Efficient Noninvasive Genetic Monitoring of Brown Bears (Ursus arctos)".PLOS ONE 11.11(2016):e0165259. |
入库方式： OAI收割
来源：昆明动物研究所
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