五步蛇出血毒结构和功能的研究
文献类型：学位论文
| 作者 | 康如钰 |
| 学位类别 | 硕士 |
| 答辩日期 | 1994 |
| 授予单位 | 中国科学院研究生院 |
| 授予地点 | 北京 |
| 导师 | 冉永禄 |
| 关键词 | 五步蛇 出血毒素 血小板聚集抑制 |
| 其他题名 | Purification and Characterizationof Two Hemrrhagic Toxins from the Venom of Agkistrodon Acutus |
| 中文摘要 | 通过Sephadex-G-100分子筛层析，QAE-Sephadex-A-50阴离子交换层析和两次Poly-buffer-exchanger离子交换层析，分得两个出血毒蛋白，分别称之为HF-1和HF-2。通过碱性PAGE，等电聚焦（IEF），SDS-PAGE和线性密度梯度PAGE，证实两出血蛋白为电泳均一。它们均为三聚体蛋白，分别含有三个相同亚基。HF-1含3 x 113个氨基酸位点，而HF-2含3 x 104个氨基酸位点。EDTA可抑制两者的活性，而碘代乙酸部份抑制HF-2的活性而不抑制HF-1的活性。从氨基酸组成分析发现，HF-1不含有Cys，而HF-1的激光喇曼光谱也没有Cys的特征峰，这在出血毒中是少见的。HF-1和HF-2均属#alpha#－纤溶酶类，同时兼有出血活性和蛋白水解酶活性。还实验了出血HF-2对血小板聚焦的抑制活性，证实HF-2抑制血小板聚集依赖于其对血浆纤原的降解。 |
| 英文摘要 | Two hemorrhagic toxins from the venom of Agkistrodon acutus are purified by gel filtration on Sehadex-G-100 and ionexchange chromatography on DEAE-Sephadex-A-50 and twice poly-buffer-exchanger. They are both trimers and homogeneous judged by IEF and liear density gradient PAGE. Each of them has three homo-subunits. EDTA could inhibit the hemorrhagic activity of them, however, Cysteine could partially inhibit the activity of HF-2 but not that of HF-1. The effect of HF-2 on platelet aggregationis studied and it is demonstrated the inhibitionof HF-2 on platelet aggregation was due to the degradation of the Aa-chain of fibrinogen. Laser Raman Spectra is used to examine the comformation of HF-1 and HF-2. The total comformation of them is #beta#-structure. The secondary content is caculated by the methods of Williams. The structure unit is divided into six kinds. |
| 语种 | 中文 |
| 公开日期 | 2010-10-15 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6216]  |
| 专题 | 昆明动物研究所_其他 |
| 推荐引用方式 GB/T 7714 | 康如钰. 五步蛇出血毒结构和功能的研究[D]. 北京. 中国科学院研究生院. 1994. |
入库方式： OAI收割
来源：昆明动物研究所
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