新生豚鼠高胆红素血症时P50抑制变化的研究
文献类型：期刊论文
| 作者 | 丁辉1; 何平2; 马原野3 |
| 刊名 | 蚌埠医学院学报
 |
| 出版日期 | 2007 |
| 卷号 | 32期号:1页码:10-13 |
| 关键词 | 胆红素过多 黄疸,新生儿 P50 感觉门控 豚鼠 |
| 其他题名 | A study on the change of sensory gating in guinea pigs with hyperbilirubinemia |
| 合作状况 | 其它 |
| 中文摘要 | 目的:评价新生豚鼠高胆红素血症时P50抑制值[即T/C值(T为实验,C为对照)]的动态变化对神经系统的毒性作用.方法:出生5～7天的新生豚鼠30只,随机分成5组,每组6只.其中,第一组为正常对照组(C),其余4组为实验组(T).5组新生豚鼠均在硫喷妥钠麻醉下行颅骨电极包埋术,待手术麻醉清醒后,分别测各组新生豚鼠的T/C值.检测完毕,分别向其中2组实验组动物腹腔注入胆红素溶液100 μg/g,4 h、8 h后观察;另2组实验组动物腹腔注入胆红素溶液200 μg/g,4 h、8 h后观察.正常对照组动物均向腹腔注入生理盐水0.5 ml.各组动物在观察完行为学变化和T/C值检测后,再迅速处死动物,取脑组织,在光镜、电镜下观察脑组织结构的变化.结果:实验豚鼠在注入胆红素溶液后,除Tlb组变化不明显外(P＞0.05),其余各组豚鼠T/C值的变化差异均有统计学意义(P＜0.01).不同实验组与正常对照组的T/C值的变化差异也均有统计学意义(P＜0.01).结论:P50抑制(T/C)在高胆红素血症不同时段均有明显变化,可以较早期地(在胆红素聚集阶段)预测胆红素对神经系统的毒性作用,为临床预防和评价高胆红素血症对新生儿神经系统损伤提供一种新的方法. |
| 英文摘要 | Objective：To evaluate the toxic influence of the change of T/C on the nervous system of neonatal guinea pigs with hyperbilirubinemia. Methods：Thirty neonatal guinea pigs at 5 to 7 days of age were randomly divided into five equal groups：one control group and four experiment groups, were embedded electrode in cranium after anesthesia. Experiment groups were injected with crystal bilirubin （T1,100 μg/g;T2,200μg/g） ,and control group with normal saline（0.5 ml）,T/C were recorded and nervous systems including their behavior were observed before and after 4 h, 8 h of injection.Then guinea pigs were executed and taken their brain tissue ,which were observed under optical and electron microscope. Results： T/C in experiment groups ： there is statistical significance between before and after celiac injection of bilirubin （ P 〈 0.01 ）, except T1 b group （ P 〉 0.05 ）. T/C in all groups ： T1 （ T1 a, T1 b ） groups and T2（ T2a, T2b） groups respectively compared with the control group and there is statistical significance between them（ P 〈 0.01 ）. Conclusions：The change of T/C in hyperbilirubinemia at different concentration and time ,we can forecast the injury of nerval toxicity in hyperbilirubinemia early. Sensory gating, as a measurement of cognize and attention to the brain, may evaluate and prevent the injury of brain to neonate in clinic. |
| 收录类别 | 其他 |
| 资助信息 | 云南省教育厅自然科学研究资助项目(03z520c). |
| 语种 | 中文 |
| 公开日期 | 2010-08-06 |
| 源URL | [http://159.226.149.42:8088/handle/152453/1036]  |
| 专题 | 昆明动物研究所_认知障碍病理学 |
| 作者单位 | 1.武警安徽总队医院儿科,安徽合肥230041 2.昆明医学院附属二院儿科,云南昆明650031 3.中国科学院昆明动物研究所;云南昆明650223 |
| 推荐引用方式 GB/T 7714 | 丁辉,何平,马原野. 新生豚鼠高胆红素血症时P50抑制变化的研究[J]. 蚌埠医学院学报,2007,32(1):10-13. |
| APA | 丁辉,何平,&马原野.(2007).新生豚鼠高胆红素血症时P50抑制变化的研究.蚌埠医学院学报,32(1),10-13. |
| MLA | 丁辉,et al."新生豚鼠高胆红素血症时P50抑制变化的研究".蚌埠医学院学报 32.1(2007):10-13. |
入库方式： OAI收割
来源：昆明动物研究所
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