Melatonin treatment increases the transcription of cell proliferation-related genes prior to inducing cell death in C6 glioma cells in vitro
文献类型：期刊论文
| 作者 | Qu JG1,2; Rizak JD2; Li XM1,2; Li JJ3; Ma YY[*]2,4 |
| 刊名 | ONCOLOGY LETTERS
 |
| 出版日期 | 2013 |
| 卷号 | 6期号:2页码:347-352 |
| 关键词 | melatonin glioma Nestin Bmi-1 Sox2 cell viability survival |
| 通讯作者 | yuanma0716@vip.sina.com |
| 合作状况 | 其它 |
| 中文摘要 | A number of studies have suggested that melatonin possesses anticancer properties. However, conflicting data exists with regard to the role of melatonin in the treatment of cancer. In the present study, the effects of melatonin on the transcriptional regulation of three genes associated with cell proliferation (Nestin, Bmi-1 and Sox2), and on C6 glioma cell survival and viability, were investigated in vitro to evaluate the use of melatonin in cancer therapy. Melatonin was shown to increase the mRNA levels of Nestin, Bmi-1 and Sox2 in a similar pattern, with the highest mRNA levels noted at a concentration of 3 mM. At higher concentrations of melatonin (5 mM), the mRNA levels of Nestin, Bmi-1 and Sox2 were reduced from their peak levels, and were correlated with changes observed in immunofluorescence morphology studies, cell viability and survival assays. Immunofluorescence studies of Nestin-stained cells demonstrated that treatment with a higher concentration of melatonin (3 and 5 mM) led to the Nestin filaments condensing and rearranging around the cell nuclei, and an alteration in the cell morphology. C6 cell viability was also significantly decreased at 3 mM melatonin, and cell death was observed at 5 and 10 mM melatonin. These results suggested that Nestin, Bmi-1 and Sox2 were strongly correlated with the survival of C6 cells following treatment with melatonin, and that high therapeutic concentrations of melatonin (>5 mM) were required to induce cell death. These findings suggested that the implementation of melatonin in the treatment of glioma and other types of cancer may be inhibited by conflicting cell growth signals in cells. Therefore, adjunct therapy is required to improve the efficacy of melatonin in the treatment of cancer. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (grant no. KSCX2- EW-J-23). |
| 语种 | 英语 |
| WOS记录号 | WOS:000322381000009 |
| 公开日期 | 2013-08-30 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7621]  |
| 专题 | 昆明动物研究所_认知障碍病理学 |
| 作者单位 | 1.School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026 2.State Key Laboratories of Brain and Cognitive Science,Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 3.Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 4.State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China |
| 推荐引用方式 GB/T 7714 | Qu JG,Rizak JD,Li XM,et al. Melatonin treatment increases the transcription of cell proliferation-related genes prior to inducing cell death in C6 glioma cells in vitro[J]. ONCOLOGY LETTERS,2013,6(2):347-352. |
| APA | Qu JG,Rizak JD,Li XM,Li JJ,&Ma YY[*].(2013).Melatonin treatment increases the transcription of cell proliferation-related genes prior to inducing cell death in C6 glioma cells in vitro.ONCOLOGY LETTERS,6(2),347-352. |
| MLA | Qu JG,et al."Melatonin treatment increases the transcription of cell proliferation-related genes prior to inducing cell death in C6 glioma cells in vitro".ONCOLOGY LETTERS 6.2(2013):347-352. |
入库方式： OAI收割
来源：昆明动物研究所
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