Transplantable neural progenitor populations derived from Rhesus monkey embryonic stem cells
文献类型：期刊论文
| 作者 | Li TQ1,2,3; Zheng JW3,4; Li J1; Jin LF1,2,3; Ma YY2,4; Wolf DP5; Zhou Q[*]6; Ji WZ[*]1,2; Xie YH1,2; Wang SF1,2,3 |
| 刊名 | STEM CELLS
 |
| 出版日期 | 2005 |
| 卷号 | 23期号:9页码:1295-1303 |
| 关键词 | Rhesus monkey embryonic stem cells neural progenitors hepatocyte growth factor G5 supplement differentiation |
| 通讯作者 | qzhou@ioz.ac.cn ; wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Cell-based therapies using embryonic stem cells (ESCs) in the treatment of neural disease will require the generation of homogenous donor neural progenitor (NP) populations. Here we describe an efficient culture system containing hepatocyte growth factor (HGF) and G5 supplement for the production of highly enriched (88.3% +/- 8.1%)populations of NPs from rhesus monkey ESCs. Additional purification resulted in NP preparations that were 98% nestin positive. Moreover, NPs, as monolayers or neurospheres, could be maintained for prolonged periods of time in media containing HGF+G5 or G5 alone. In vitro differentiation and in vivo transplantation assays showed that NPs could differentiate into neurons, astrocytes, and oligodendrocytes. The kinds and quantities of differentiated cells derived from NPs were closely correlated with their niches in vivo. Glial differentiation was predominant in periventricular areas, whereas cells migrating into the cortex were mostly neurons. Cell counts showed that 2 months after transplantation, approximately 25% of transplanted NPs survived and 65% - 80% of the surviving transplanted cells migrated along the ventricular wall or in a radial fashion. Subcloning demonstrated that several clonal lines derived from NPs expressed nestin and differentiated into three neural lineages in vitro and in rat brains in vivo. In contrast, some subcloned lines showed restricted differentiation both in vitro and in vivo in rat brains. These observations set the stage for obtaining highly enriched NPs and evaluating the efficacy of NP-based transplantation therapy in the nonhuman primate and will provide a platform for probing the molecular mechanisms that control neural induction. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by research grants from Major State Research Development Program 2004CCA01300, G200016108, and 2001cb510100; The Chinese Academy of Sciences KSCX1- 05; Chinese National Science Foundation 30370166; and Yunnan Nature Science Foundation 2001C0009Z. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10596]  |
| 专题 | 昆明动物研究所_认知障碍病理学 昆明动物研究所_生殖与发育生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Department of Reproduction and Development, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, China 2.Kunming Primate Research Center, The Chinese Academy of Sciences, Kunming, Yunnan, China 3.Graduate School, The Chinese Academy of Sciences, Beijing, China 4.Section of Cognitive Brain Research, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, China 5.Oregon National Primate Research Center, Portland, Oregon, USA 6.Institute of Zoology, The Chinese Academy of Sciences, Beijing, China |
| 推荐引用方式 GB/T 7714 | Li TQ,Zheng JW,Li J,et al. Transplantable neural progenitor populations derived from Rhesus monkey embryonic stem cells[J]. STEM CELLS,2005,23(9):1295-1303. |
| APA | Li TQ.,Zheng JW.,Li J.,Jin LF.,Ma YY.,...&Zhang XZ.(2005).Transplantable neural progenitor populations derived from Rhesus monkey embryonic stem cells.STEM CELLS,23(9),1295-1303. |
| MLA | Li TQ,et al."Transplantable neural progenitor populations derived from Rhesus monkey embryonic stem cells".STEM CELLS 23.9(2005):1295-1303. |
入库方式： OAI收割
来源：昆明动物研究所
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