Efficient Generation of Corticofugal Projection Neurons from Human Embryonic Stem Cells
文献类型：期刊论文
| 作者 | Zhu XQ1,2,3,4; Ai ZY2,3; Hu XT[*]4; Li TQ[*]2,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:X页码:e28572. |
| 通讯作者 | xthu@mail.kiz.ac.cn |
| 英文摘要 | Efforts to study development and function of corticofugal projection neurons (CfuPNs) in the human cerebral cortex for health and disease have been limited by the unavailability of highly enriched CfuPNs. Here, we develop a robust, two-step process for generating CfuPNs from human embryonic stem cells (hESCs): directed induction of neuroepithelial stem cells (NESCs) from hESCs and efficient differentiation of NESCs to about 80% of CfuPNs. NESCs or a NESC faithfully maintain unlimitedly self-renewal and self-organized abilities to develop into miniature neural tube-like structures. NESCs retain a stable propensity toward neuronal differentiation over culture as fate-restricted progenitors of CfuPNs and interneurons. When grafted into mouse brains, NESCs successfully integrate into the host brains, differentiate into CfuPNs and effectively reestablish specific patterns of subcortical projections and synapse structures. Efficient generation of CfuPNs in vitro and in vivo will facilitate human cortex development and offer sufficient CfuPNs for cell therapy. |
| 收录类别 | SCI |
| 资助信息 | This work was funded by grants from the Chinese Ministry of Science and Technology 973 program (2012CBA01300), the Key Technologies Research and Development Program of China (2014BAI03B01), the National Natural Science Foundation of China (31360231), and Yunnan Innovation Talents of Science and Technology (2014HB054). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10222]  |
| 专题 | 昆明动物研究所_神经系统编码 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230000, PR China 2.Yunnan Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China 3.Kunming Ennovate Institute of Bioscience, Kunming, Yunnan 650500, China 4.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China |
| 推荐引用方式 GB/T 7714 | Zhu XQ,Ai ZY,Hu XT[*],et al. Efficient Generation of Corticofugal Projection Neurons from Human Embryonic Stem Cells[J]. SCIENTIFIC REPORTS,2016,6(X):e28572.. |
| APA | Zhu XQ,Ai ZY,Hu XT[*],&Li TQ[*].(2016).Efficient Generation of Corticofugal Projection Neurons from Human Embryonic Stem Cells.SCIENTIFIC REPORTS,6(X),e28572.. |
| MLA | Zhu XQ,et al."Efficient Generation of Corticofugal Projection Neurons from Human Embryonic Stem Cells".SCIENTIFIC REPORTS 6.X(2016):e28572.. |
入库方式： OAI收割
来源：昆明动物研究所
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