Resveratrol Attenuates Formaldehyde Induced Hyperphosphorylation of Tau Protein and Cytotoxicity in N2a Cells
文献类型：期刊论文
| 作者 | He XP1,2,3; Li ZH1,2; He RQ4,5; Su M3; Qin DD[*]1; Wang JK[*]3; Hu XT[*]1,6,7; Rizak JD1; Wu SH1,2; Wang WB1 |
| 刊名 | Frontiers in Neuroscience
 |
| 出版日期 | 2017 |
| 卷号 | 10期号:X页码:e598 |
| 关键词 | Alzheimer's disease CaMKII GSK-3β PP2A formaldehyde resveratrol tau protein |
| 通讯作者 | qindong108@163.com ; wjkyimm@163.com ; xthu@mail.kiz.ac.cn |
| 英文摘要 | Recent studies have demonstrated that formaldehyde (FA)-induced neurotoxicity is important in the pathogenesis of Alzheimer's disease (AD). Elevated levels of FA have been associated with memory impairments and the main hallmarks of AD pathology, including β-amyloid plaques, tau protein hyperphosphorylation, and neuronal loss. Resveratrol (Res), as a polyphenol anti-oxidant, has been considered to have therapeutic potential for the treatment of AD. However, it has not been elucidated whether Res can exert its neuroprotective effects against FA-induced neuronal damages related to AD pathology. To answer this question, the effects of Res were investigated on Neuro-2a (N2a) cells prior to and after FA exposure. The experiments found that pre-treatment with Res significantly decreased FA-induced cytotoxicity, reduced cell apoptosis rates, and inhibited the hyperphosphorylation of tau protein at Thr181 in a dose-dependent manner. Further tests revealed that this effect was associated with the suppression of glycogen synthase kinase (GSK-3β) and calmodulin-dependent protein kinase II (CaMKII) activities, both of which are important kinases for tau protein hyperphosphorylation. In addition, Res was found to increase the activity of phosphoseryl/phosphothreonyl protein phosphatase-2A (PP2A). In summary, these findings provide evidence that Res protects N2a cells from FA-induced damages and suggests that inhibition of GSK-3β and CaMKII and the activation of PP2A by Res protect against the hyperphosphorylation and/or mediates the dephosphorylation of tau protein, respectively. These possible mechanisms underlying the neuroprotective effects of Res against FA-induced damages provide another perspective on AD treatment via inhibition of tau protein hyperhosphorylation. |
| 资助信息 | This research was supported by the National Program for Key Basic Research Projects (973 Programs 2015CB755605, 2012CB825503, 2012CBA01304), the Strategic Priority Research Program of the CAS (XDB02020005), the Key Research Program of the Chinese Academy of Sciences, the Selected Frontier Scientific Significant Breakthrough Project of the CAS, the Key Program of the Chinese Academy of Sciences (KZCC-EW- 103-2), the Training Program of the Major Research Plan of the National Natural Science Foundation of China (91332120), the National Natural Science Foundation of China (81471312, 31271167, 81271495, 81460352), the Yunnan Provincial Project to Attract One-hundred Exceptional Talents From Overseas and the Applied Basic Research Programs of Science and Technology Commission Foundation of Yunnan Province (2014FA047). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10868]  |
| 专题 | 昆明动物研究所_神经系统编码 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Nerve System Coding Discipline Group, Kunming College of Life Science, University of the Chinese Academy of Sciences, Kunming, China 3.Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation, Yunnan Bai Yao Group Innovation and R&D Center, Yunnan Institute of Materia Medica, Kunming, China 4.State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China 5.Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China 6.CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China 7.Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China |
| 推荐引用方式 GB/T 7714 | He XP,Li ZH,He RQ,et al. Resveratrol Attenuates Formaldehyde Induced Hyperphosphorylation of Tau Protein and Cytotoxicity in N2a Cells[J]. Frontiers in Neuroscience,2017,10(X):e598. |
| APA | He XP.,Li ZH.,He RQ.,Su M.,Qin DD[*].,...&Wang WB.(2017).Resveratrol Attenuates Formaldehyde Induced Hyperphosphorylation of Tau Protein and Cytotoxicity in N2a Cells.Frontiers in Neuroscience,10(X),e598. |
| MLA | He XP,et al."Resveratrol Attenuates Formaldehyde Induced Hyperphosphorylation of Tau Protein and Cytotoxicity in N2a Cells".Frontiers in Neuroscience 10.X(2017):e598. |
入库方式： OAI收割
来源：昆明动物研究所
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