Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells
文献类型：期刊论文
| 作者 | Chen DL1,2,3; Li RR1,2,3; Zheng J1,2,3; Lu B1,2,3; Wei Q1,2,3; Wang SF1,2,3; Xie YH1,3; JI WZ[*]1,3 |
| 刊名 | 动物学研究
 |
| 出版日期 | 2009 |
| 卷号 | 30期号:4页码:345−353 |
| 关键词 | Embryonic stem cells Rhesus monkey feeders Self-renewal Pluripotency Expression profile |
| 其他题名 | 五株同源饲养层支持猕猴胚胎干细胞能力的比较 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 中文摘要 | 我们以前的研究建立了五株猕猴饲养层细胞系来支持猕猴胚胎干细胞(rESCs)的生长:一岁猴耳皮肤成纤维细胞(MESFs)、两岁猴输卵管成纤维细胞(MOFs)、成年猴卵泡颗粒成纤维样细胞(MFGs)、成年猴卵泡颗粒上皮样细胞(MFGEs),以及 MESFs 的克隆成纤维细胞(CMESFs)。我们发现 MESFs、CMESFs、MOFs 和 MFGs,而不是 MFGEs 支持猕猴胚胎干细胞(rESCs, rhesus embryonic stem cells)的生长。通过半定量 PCR 的方法,我们在支持性的饲养层细胞中检测到了一些基因的高表达。在本研究中,我们运用 Affymetrix 公司的 GeneChip Rhesus Macaque Genome Array 芯片来研究这五株同源饲养层的表达谱,希望发现哪些细胞因子和信号通路在维持 rESCs中起到重要作用。结果表明,除 MFGE 外,包括 GREM2、 bFGF,、KITLG,、DKK3、GREM1、AREG、SERPINF1 和 LTBP1 等八个基因的 mRNA 在支持性的饲养层细胞中高表达。本研究结果提示,很多信号通路在支持 rESCs的未分化生长和多潜能性方面可能起到了冗余的作用。 |
| 英文摘要 | In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts (MESFs), clonally derived fibroblasts from the MESFs (CMESFs), monkey oviductal fibroblasts (MOFs), monkey follicular granulosa fibroblast-like (MFGs) cells, monkey follicular granulosa epithelium-like (MFGEs) cells, were developed for the maintenance of rhesus embryonic stem cells (rESCs). We found that MESFs, CMESFs, MOFs and MFGs, but not MFGEs, support the growth of rhesus embryonic stem cells. Moreover, we detected some genes that are upregulated in supportive feeder cell lines by semi-quantitative PCR. In the present study, we applied the GeneChip® Rhesus Macaque Genome Array of Affymetrix Corporation to study the expression profiles of these five feeder cell lines, in purpose to find out which cytokines and signaling pathways were important in maintaining the rESCs. mRNAs of eight genes, including GREM2, bFGF, KITLG, DKK3, GREM1, AREG, SERPINF1 and LTBP1, were found to be upregulated in supportive feeder cell lines, but not in MFGE. The results indicate that many signaling pathways may play redundant roles in supporting the undifferentiated growth and maintenance of pluripotency in rESCs. |
| 收录类别 | 其他 |
| 资助信息 | 973 计划(2006CB701505；2007CB947701)；中科院创新工程试点经费(KSCX2-YW-R-47)；国家自然科学基金(30570906； 30700159；30700425) |
| 语种 | 英语 |
| 公开日期 | 2010-08-03 |
| 源URL | [http://159.226.149.42:8088/handle/152453/832]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Kunming Primate Research Center and Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China 3.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Chen DL,Li RR,Zheng J,et al. Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells[J]. 动物学研究,2009,30(4):345−353. |
| APA | Chen DL.,Li RR.,Zheng J.,Lu B.,Wei Q.,...&JI WZ[*].(2009).Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells.动物学研究,30(4),345−353. |
| MLA | Chen DL,et al."Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells".动物学研究 30.4(2009):345−353. |
入库方式： OAI收割
来源：昆明动物研究所
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