藏獒血液蛋白遗传多样性的研究
文献类型：期刊论文
| 作者 | 兰小平1; 陈永昌2; 郭宪3; 鄢珣4; 崔泰保4 |
| 刊名 | 湖北农业科学
 |
| 出版日期 | 2009 |
| 卷号 | 48期号:12页码:2938-2940 |
| 关键词 | 藏獒 遗传多样性 血液蛋白多态性 遗传距离 |
| 其他题名 | Studies on Genetic Diversity of Tibetan Mastiff Using Blood Protein Polymorphism |
| 通讯作者 | xplan74@163.com |
| 合作状况 | 其它 |
| 中文摘要 | 采用不连续垂直板聚丙烯酰胺凝胶电泳技术,对河曲藏獒、青海藏獒、青海藏狮犬和青海土种犬共4个群体103只犬的9个血液蛋白基因座(Tf、Po、Es-1、Es-2、Sα2、Hb、Alb、Pr、Amy)的多态性进行了检测,分析了两个藏獒群体的群体内和群体间的遗传变异,并以Nei氏标准遗传距离(D)为基础用UPGMA法探讨了不同犬群之间的遗传关系。结果表明,在4个被测犬群中,Tf、Po、Es-1、Es-2和Sα25个基因座上存在多态性,其中Tf、Es-1和Po分别由3个等位基因所控制,Es-2和Sα2分别由2个等位基因所控制,而Hb、Alb、Pr和Amy基因座均呈现单态;河曲藏獒群体内遗传变异较青海藏獒丰富,而两个藏獒群体间的遗传分化程度很低(GST=0.0187);以Nei氏标准遗传距离(D)为基础的UPGMA法聚类结果表明,青海藏獒与青海藏狮犬和青海土种犬的遗传关系近于河曲藏獒。 |
| 英文摘要 | 103 blood samples were taken from four populations of Hequ Tibetan mastiff, Qinghai Tibetan mastiff, Tibetan spaniel and native dogs of Qinghai. Nine blood protein loci （Tf,Po,Es-1 ,Es-2,Sα2 ,Hb,Alb,Pr,Amy） were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Genetic variations were analyzed within and between two populations of Tibetan mastiff. The genetic relationship between dog populations were analyzed by Nei＇s standard genetic distance （D） and UPGMA. The results showed that genetic variations were observed in Tf, Po,Es-1,Es-2,and Sα2, others were not polymorphic. There were three alleles at the loci of Tf, Es-1 and Po, two alleles at the loci of Es-2 and Sα2. The genetic diversities within population of Hequ Tibetan mastiff were richer than that of Qinghai Tibetan mastiff. There was less variation among Tibetan mastiff populations and the genetic differentiation was very limited between Tibetan mastiff populations （GST=0.018 7）; A dendrogram by Nei＇s standard genetic distance （D） and UPGMA showed that genetic relationship between Hequ Tibetan mastiff and Qinghai Tibetan mastiff was distant. |
| 收录类别 | 其他 |
| 资助信息 | 甘肃省科技厅基金资助项目(GKC-97-27-5);天水师范学院青年基金项目(X4-25). |
| 语种 | 中文 |
| 公开日期 | 2010-08-04 |
| 源URL | [http://159.226.149.42:8088/handle/152453/856]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.天水师范学院生命科学与化学学院，甘肃天水 741000 2.中国科学院昆明动物研究所，昆明650223 3.中国农业科学院兰州畜牧与兽药研究所，兰州730050 4.甘肃农业大学，兰州 730070 |
| 推荐引用方式 GB/T 7714 | 兰小平,陈永昌,郭宪,等. 藏獒血液蛋白遗传多样性的研究[J]. 湖北农业科学,2009,48(12):2938-2940. |
| APA | 兰小平,陈永昌,郭宪,鄢珣,&崔泰保.(2009).藏獒血液蛋白遗传多样性的研究.湖北农业科学,48(12),2938-2940. |
| MLA | 兰小平,et al."藏獒血液蛋白遗传多样性的研究".湖北农业科学 48.12(2009):2938-2940. |
入库方式： OAI收割
来源：昆明动物研究所
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