Vitrification of Yunnan Yellow Cattle oocytes: work in progress
文献类型：期刊论文
| 作者 | Li XL1; Su L1; Li YH1; Ji WZ[*]1; Dinnyes A2 |
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 58期号:7页码:1253-1260 |
| 关键词 | bovine oocyte cryopreservation OPS SSV |
| ISSN号 | 0093-691X |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The objective of this study was to provide a simple cryopreservation method for oocytes from Yunnan Yellow Cattle and facilitate preservation efforts in this native Chinese breed, which is threatened by agricultural modernization. Cumulus-oocyte complexes (COCs) were collected from slaughterhouse ovaries and matured in vitro for 22-24 h, then selected for cryopreservation. Vitrification in open pulled straws (OPS) or in microdrops on a cooled metal surface (solid surface vitrification, SSV) was compared. The OPS vitrification solution consisted of 20% ethylene glycol (EG) and 20% DMSO. The SSV solution was a mixture of 35% EG, 5% polyvinyl-pyrrolidon (PVP) and 0.4 M trehalose. Vitrified and warmed oocytes were either fertilized in vitro or parthenogenetically activated. The rates of cleavage and development to blastocysts of fertilized oocytes following OPS versus SSV were not statistically different (38.3 and 12.5% versus 35.8 and 6.0%, respectively). The corresponding rates of parthenogenetic development to blastocysts were also not different (8.2 versus 3.5%, respectively). Development to blastocysts of non-vitrified controls following fertilization was significantly higher than that of the vitrified oocytes (22.6%, P < 0.05). These results demonstrate for the first time, that although both OPS and SSV procedures reduced embryonic development, Yunnan Yellow Cattle oocytes are capable of developing to blastocysts following cryopreservation. |
| 收录类别 | SCI |
| 原文出处 | 2002581253.pdf |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5551]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China 2.Hungarian Acad Sci, Res Grp Anim Breeding, H-2103 Godollo, Hungary |
| 推荐引用方式 GB/T 7714 | Li XL,Su L,Li YH,et al. Vitrification of Yunnan Yellow Cattle oocytes: work in progress[J]. THERIOGENOLOGY,2002,58(7):1253-1260. |
| APA | Li XL,Su L,Li YH,Ji WZ[*],&Dinnyes A.(2002).Vitrification of Yunnan Yellow Cattle oocytes: work in progress.THERIOGENOLOGY,58(7),1253-1260. |
| MLA | Li XL,et al."Vitrification of Yunnan Yellow Cattle oocytes: work in progress".THERIOGENOLOGY 58.7(2002):1253-1260. |
入库方式： OAI收割
来源：昆明动物研究所
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