Serum-free culture of rhesus monkey embryonic stem cells
文献类型：期刊论文
| 作者 | Pei Y1,2; Ma J1; Zhang X1,2; Ji W[*]1 |
| 刊名 | ARCHIVES OF ANDROLOGY
 |
| 出版日期 | 2003 |
| 卷号 | 49期号:5页码:331-342 |
| 关键词 | culture embryonic stem cell rhesus monkey serum-free medium |
| ISSN号 | 0148-5016 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Previous studies have shown that the maintenance and proliferation of undifferentiated rhesus monkey embryonic stem (rES) cells requires medium supplemented with fetal bovine serum (FBS). Due to the uncharacterized composition and variation in serum nature, the present study aimed to replace the serum-containing medium with a serum-free medium in the rES cell culture. The results showed that after the initial 48-h culture in the routinely used serum-containing medium, rES cells can grow and proliferate for a prolonged period in the serum-free medium composed of DMEM supplemented with a cocktail of BSA, IGF-1, TGF-alpha, bFGF, aFGF, estradiol, and progesterone. rES cells cultured in the serum-free medium maintained high level of alkaline phosphatase activity and OCT4 level. There was no indication of differentiation as judged by the marker gene expression of all three embryonic germ layers and trophoblast. In addition, serum-free culture would not affect the passage capacity and differentiation potential of rES cells. This work will facilitate the future study of induced differentiation of rES cells and other applications. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Major State Research Development Program (2001CB509905). |
| 原文出处 | 200349331.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5563]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan, China 2.Graduate School, the Chinese Academy of Sciences, Beijing, China |
| 推荐引用方式 GB/T 7714 | Pei Y,Ma J,Zhang X,et al. Serum-free culture of rhesus monkey embryonic stem cells[J]. ARCHIVES OF ANDROLOGY,2003,49(5):331-342. |
| APA | Pei Y,Ma J,Zhang X,&Ji W[*].(2003).Serum-free culture of rhesus monkey embryonic stem cells.ARCHIVES OF ANDROLOGY,49(5),331-342. |
| MLA | Pei Y,et al."Serum-free culture of rhesus monkey embryonic stem cells".ARCHIVES OF ANDROLOGY 49.5(2003):331-342. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。