Effect of amino acids on cryopreservation of cynomolgus monkey (Macaca fascicularis) sperm
文献类型:期刊论文
作者 | Li YH1,2,3; Si W1,2; Zhang XZ1,2; Dinnyes A4; Ji WZ[*]1 |
刊名 | AMERICAN JOURNAL OF PRIMATOLOGY
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出版日期 | 2003 |
卷号 | 59期号:4页码:159-165 |
关键词 | amino acid sperm cryopreservation Macaca fascicularis |
ISSN号 | 0275-2565 |
通讯作者 | wji@mail.kiz.ac.cn |
合作状况 | 其它 |
英文摘要 | The effects of three amino acids (proline, glutamine, and glycine) added to the freezing medium Tes-Tris-egg yolk (TTE) for cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa were studied. This is the first report on the effects of amino acids on nonhuman primate sperm cryopreservation. The addition of 5 mM proline, 10 mM glutamine, and 10 or 20 mM glycine each significantly improved post-thaw sperm motility and membrane and acrosome integrity compared with the control (TTE alone). However, a significant decrease in motility and membrane/acrosome integrity was observed when amino acid concentrations increased to 60 mM for proline and glutamine, and 80 mM for glycine. The results suggest that adding a limited amount of amino acids to the freezing media is beneficial for freezing cynomolgus monkey sperm. |
收录类别 | SCI |
资助信息 | China National Science Foundation; Contract grant number: 39970117; Chinese Academy of Sciences; Contract grant number: KZ952-J1-109. |
原文出处 | 200359159.pdf |
公开日期 | 2010-08-24 |
源URL | [http://159.226.149.42:8088/handle/152453/5565] ![]() |
专题 | 昆明动物研究所_生殖与发育生物学 |
作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People’s Republic of China 2.Graduate School, Chinese Academy of Sciences, Beijing, People’s Republic of China 3.College of Food Science and Technology, Yunnan Agricultural University, Kunming Yunnan, People’s Republic of China 4.Research Group for Applied Animal Genetics and Biotechnology, Hungarian Academy of Sciences and Szent Istvan University, Godollo, Hungary |
推荐引用方式 GB/T 7714 | Li YH,Si W,Zhang XZ,et al. Effect of amino acids on cryopreservation of cynomolgus monkey (Macaca fascicularis) sperm[J]. AMERICAN JOURNAL OF PRIMATOLOGY,2003,59(4):159-165. |
APA | Li YH,Si W,Zhang XZ,Dinnyes A,&Ji WZ[*].(2003).Effect of amino acids on cryopreservation of cynomolgus monkey (Macaca fascicularis) sperm.AMERICAN JOURNAL OF PRIMATOLOGY,59(4),159-165. |
MLA | Li YH,et al."Effect of amino acids on cryopreservation of cynomolgus monkey (Macaca fascicularis) sperm".AMERICAN JOURNAL OF PRIMATOLOGY 59.4(2003):159-165. |
入库方式: OAI收割
来源:昆明动物研究所
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