Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa
文献类型：期刊论文
| 作者 | Li YH1,2,3; Cai KJ1; Kovacs A4; Ji WZ[*]1 |
| 刊名 | JOURNAL OF ANDROLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:3页码:387-395 |
| 关键词 | Sperm freezing motility plasma membrane acro- some permeability coefficient |
| ISSN号 | 0196-3635 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The cryoprotective effects of 11 different extenders, TTE, DM, mDM, LG-DM, G-DM, TCG, TEST, TSM, Test-M, Test-H, and LM, on sperm cryopreservation of cynomolgus monkey (Macaca fascicularis) have been compared with glycerol as cryoprotectant. Sperm motility, plasma membrane, and acrosomal integrity were examined to evaluate frozen-thawed sperm function. The results showed that TTE, DM, mDM, LG-DM, G-DM, and TCG exhibited the best and similar protective efficiencies for cynomolgus monkey sperm cryopreservation in terms of sperm motility and plasma membrane integrity (P > .05). The acrosomal integrity for spermatozoa cryopreserved in TCG was statistically lower than that of TTE, DM, mDM, LG-DM, and G-DM (P < .05) but was significantly higher than that of TEST, TSM, Test-M, Test-H, and LM (P < .05). The postthaw sperm motility for 5 other extenders (TEST, TSM, Test-M, Test-H, and LM) did not exceed 30%, and the 3 sperm parameters evaluated for them were significantly lower than that of TTE, DM, mDM, LG-DM, G-DM, and TCG (P < .05). On the basis of these findings, 5 commonly used permeating cryoprotectants, glycerol, ethylene glycol, dimethyl sulfoxide, acetamide and propylene glycol have further been tested for their effectiveness on sperm cryopreservation in extenders of TTE, DM, mDM, LG-DM, G-DM, and TCG. The results showed that the sperm cryoprotective efficiencies of glycerol and ethylene glycol were similar and best among 5 permeating cryoprotectant treatments (P > .05). Dimethyl sulfoxide or acetamide resulted in average cryoprotection for cynomolgus monkey spermatozoa: poorer than glycerol or ethylene glycol but better than that of propylene glycol (P < .05). In addition, the action of permeating cryoprotectant appeared to be independent of extenders. The results in the present study demonstrate that 1) TTE, DM, mDM, LG-DM, G-DM, and TCG are excellent extenders and suitable for cynomolgus monkey sperm cryopreservation; 2) the mechanism of action of permeating cryoprotectants are not affected by extender composition; 3) ethylene glycol has a similar cryoprotective efficacy to glycerol that makes it a successful cryoprotectant for sperm cryopreservation in cynomolgus monkeys. |
| 学科主题 | Andrology |
| 收录类别 | SCI |
| 资助信息 | Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166. |
| 原文出处 | 200526387.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5589]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing, China 3.College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China 4.Research Institute for Animal Breeding and Nutrition, Herceghalom, Gesztenyes, Hungary. |
| 推荐引用方式 GB/T 7714 | Li YH,Cai KJ,Kovacs A,et al. Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa[J]. JOURNAL OF ANDROLOGY,2005,26(3):387-395. |
| APA | Li YH,Cai KJ,Kovacs A,&Ji WZ[*].(2005).Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa.JOURNAL OF ANDROLOGY,26(3),387-395. |
| MLA | Li YH,et al."Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa".JOURNAL OF ANDROLOGY 26.3(2005):387-395. |
入库方式： OAI收割
来源：昆明动物研究所
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