The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm
文献类型：期刊论文
| 作者 | Niu YY1,2,3; Greube A1; Ji WZ2; Jewgenow K[*]1 |
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 66期号:4页码:989-995 |
| 关键词 | cats immunocontraception zona pellucida sperm competition |
| ISSN号 | 0093-691X |
| 通讯作者 | jewgenow@izw-berlin.de |
| 英文摘要 | The present study aimed to establish a sensitive in vitro assay to assess the binding capacity of cat spermatozoa. Cat oocytes and epididymal sperm cells were isolated from gonads and cultured for in vitro fertilization. Before fertilization, the sperm cells were incubated either in 10 mu M green dye Fluo-3-AM or 10 mu M orange dye CellTracker Orange CMTMR (Molecular Probes), respectively. After removing the dyes by washing, sperm cells stained with each dye were added to medium drops containing oocytes in various proportions and cultured for 16 h at 37 degrees C, 5% CO2. The oocytes were examined using fluorescence microscopy. Sperm bound to oocytes, and stained with different colors, were counted. When fresh epididymal sperm were mixed in at a specific proportion, the number of sperm bound to the zona pellucida (ZP) of oocytes reflected the proportion of differently colored sperm in the medium. This indicated that neither dye influenced the binding capacity of cat sperm. Mixing fresh and cryopreserved sperm, however, resulted in a higher number of fresh sperm bound to the oocyte surface in comparison to frozen-thawed sperm. Also, the pre-incubation of cat sperm cells with ZP derived peptide reduced the sperm binding capacity by 40%. In conclusion, the presented sperm competition assay allows assessment of fertilizing capacity of cat spermatozoa in vitro when a mixture of two different populations is used. The applied supravital fluorescence dyes do not affect motility and binding capacity of sperm cells and were clearly distinguishable under fluorescence microscopy. We demonstrate that the assay can be used to study the impact of sperm treatment, such as cryopreservation or pre-incubation in bioactive peptides, on fertilizing capacity. |
| 收录类别 | SCI |
| 资助信息 | This work received financial support from Max- Planck-Society and Chinese Academy of Sciences. |
| 原文出处 | 200666989.pdf |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5595]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Institute for Zoo and Wildlife Research, PF 601103, D-10252 Berlin, Germany 2.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiao Chang Dong Lu, Kunming, Yunnan 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Niu YY,Greube A,Ji WZ,et al. The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm[J]. THERIOGENOLOGY,2006,66(4):989-995. |
| APA | Niu YY,Greube A,Ji WZ,&Jewgenow K[*].(2006).The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm.THERIOGENOLOGY,66(4),989-995. |
| MLA | Niu YY,et al."The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm".THERIOGENOLOGY 66.4(2006):989-995. |
入库方式： OAI收割
来源：昆明动物研究所
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