Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm
文献类型：期刊论文
| 作者 | Xu J1; Guo Z2; Su L3; Nedambale TL1; Zhang J4; Schenk J5; Moreno JF6; Dinnyes A7; Ji W2; Tian XC4 |
| 刊名 | JOURNAL OF DAIRY SCIENCE
 |
| 出版日期 | 2006 |
| 卷号 | 89期号:7页码:2510-2518 |
| 关键词 | sorted sperm in vitro fertilization vitrification embryo transfer |
| ISSN号 | 0022-0302 |
| 通讯作者 | fuliangd@evergen.com |
| 合作状况 | 其它 |
| 英文摘要 | In vitro fertilization (IVF) is a feasible way to utilize sex-sorted sperm to produce offspring of a predetermined sex in the livestock industry. The objective of the present study was to examine the effects of various factors on bovine IVF and to systematically improve the efficiency of IVF production using sex-sorted sperm. Both bulls and sorting contributed to the variability among differential development rates of embryos fertilized by sexed sperm. Increased sorting pressures (275.8 to 344.75 kPa) did not have a significant effect on the in vitro fertility of the sorted sperm; neither did an extended period of 9 to 14 h from semen collection to sorting. As few as 600 sorted sperm were used to fertilize an oocyte, resulting in blastocyst development of 33.2%. Postwarming of vitrified sexed IVF embryos resulted in high morphological survival (96.3%) and hatching (84.4%) rates, similar to those fertilized by nonsexed sperm (93.1 and 80.6%, respectively). A 40.9% pregnancy rate was established following the transfer of 3,627 vitrified, sexed embryos into synchronized recipients. This was not different from the rates with nonsexed IVF (41.9%, n = 481), or in vivo-produced (53.1%, n = 192) embryos. Of 458 calves born, 442 (96.5%) were female and 99.6% appeared normal. These technologies (sperm sexing-IVF-vitrification-embryo transfer) provide farmers, as well as the livestock industry, with a valuable option for herd expansion and heifer replacement programs. In summary, calves were produced using embryos fertilized by sex-sorted sperm in vitro and cryopreserved by rapid cooling vitrification. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by the Small Business Innovation Research program of the USDA Cooperative State Research, Education, and Ex- tension Service (CSREES), Grant Number 2005-00178 to Fuliang Du, and Yankee Ingenuity Competition from Connecticut Innovation, Inc., to Xiangzhong Yang. |
| 原文出处 | 2006892510.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5599]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.Evergen Biotechnologies, Inc., Storrs, CT 06269 2.Xinjiang Academy of Animal Science, the Key Laboratory of Animal Biotechnology of Ministry of Agriculture, Urumqi, Xinjiang 830000, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 4.Department of Animal Science/Center for Regenerative Biology, University of Connecticut, Storrs 06269 5.XY Inc., Fort Collins, CO 80524 6.Sexing Technologies, Navasota, TX 77868 7.Department of Animal Biology, Agricultural Biotechnology Center, Godollo, Hungary |
| 推荐引用方式 GB/T 7714 | Xu J,Guo Z,Su L,et al. Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm[J]. JOURNAL OF DAIRY SCIENCE,2006,89(7):2510-2518. |
| APA | Xu J.,Guo Z.,Su L.,Nedambale TL.,Zhang J.,...&Du F[*].(2006).Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm.JOURNAL OF DAIRY SCIENCE,89(7),2510-2518. |
| MLA | Xu J,et al."Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm".JOURNAL OF DAIRY SCIENCE 89.7(2006):2510-2518. |
入库方式： OAI收割
来源：昆明动物研究所
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