中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm

文献类型:期刊论文

作者Xu J1; Guo Z2; Su L3; Nedambale TL1; Zhang J4; Schenk J5; Moreno JF6; Dinnyes A7; Ji W2; Tian XC4
刊名JOURNAL OF DAIRY SCIENCE
出版日期2006
卷号89期号:7页码:2510-2518
关键词sorted sperm in vitro fertilization vitrification embryo transfer
ISSN号0022-0302
通讯作者fuliangd@evergen.com
合作状况其它
英文摘要In vitro fertilization (IVF) is a feasible way to utilize sex-sorted sperm to produce offspring of a predetermined sex in the livestock industry. The objective of the present study was to examine the effects of various factors on bovine IVF and to systematically improve the efficiency of IVF production using sex-sorted sperm. Both bulls and sorting contributed to the variability among differential development rates of embryos fertilized by sexed sperm. Increased sorting pressures (275.8 to 344.75 kPa) did not have a significant effect on the in vitro fertility of the sorted sperm; neither did an extended period of 9 to 14 h from semen collection to sorting. As few as 600 sorted sperm were used to fertilize an oocyte, resulting in blastocyst development of 33.2%. Postwarming of vitrified sexed IVF embryos resulted in high morphological survival (96.3%) and hatching (84.4%) rates, similar to those fertilized by nonsexed sperm (93.1 and 80.6%, respectively). A 40.9% pregnancy rate was established following the transfer of 3,627 vitrified, sexed embryos into synchronized recipients. This was not different from the rates with nonsexed IVF (41.9%, n = 481), or in vivo-produced (53.1%, n = 192) embryos. Of 458 calves born, 442 (96.5%) were female and 99.6% appeared normal. These technologies (sperm sexing-IVF-vitrification-embryo transfer) provide farmers, as well as the livestock industry, with a valuable option for herd expansion and heifer replacement programs. In summary, calves were produced using embryos fertilized by sex-sorted sperm in vitro and cryopreserved by rapid cooling vitrification.
收录类别SCI
资助信息This study was supported by the Small Business Innovation Research program of the USDA Cooperative State Research, Education, and Ex- tension Service (CSREES), Grant Number 2005-00178 to Fuliang Du, and Yankee Ingenuity Competition from Connecticut Innovation, Inc., to Xiangzhong Yang.
原文出处2006892510.pdf
语种英语
公开日期2010-08-24
源URL[http://159.226.149.42:8088/handle/152453/5599]  
专题昆明动物研究所_生殖与发育生物学
作者单位1.Evergen Biotechnologies, Inc., Storrs, CT 06269
2.Xinjiang Academy of Animal Science, the Key Laboratory of Animal Biotechnology of Ministry of Agriculture, Urumqi, Xinjiang 830000, China
3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
4.Department of Animal Science/Center for Regenerative Biology, University of Connecticut, Storrs 06269
5.XY Inc., Fort Collins, CO 80524
6.Sexing Technologies, Navasota, TX 77868
7.Department of Animal Biology, Agricultural Biotechnology Center, Godollo, Hungary
推荐引用方式
GB/T 7714
Xu J,Guo Z,Su L,et al. Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm[J]. JOURNAL OF DAIRY SCIENCE,2006,89(7):2510-2518.
APA Xu J.,Guo Z.,Su L.,Nedambale TL.,Zhang J.,...&Du F[*].(2006).Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm.JOURNAL OF DAIRY SCIENCE,89(7),2510-2518.
MLA Xu J,et al."Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm".JOURNAL OF DAIRY SCIENCE 89.7(2006):2510-2518.

入库方式: OAI收割

来源:昆明动物研究所

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