Hepatocyte growth factor enhances the generation of high-purity oligodendrocytes from human embryonic stem cells
文献类型：期刊论文
| 作者 | Hua ZX[*]1,2,3; Li TQ1,2; Zhang XZ1,2; Chen YC1,2,3 |
| 刊名 | DIFFERENTIATION
 |
| 出版日期 | 2009 |
| 卷号 | 78期号:37655页码:177-184 |
| 关键词 | Human embryonic stem cells Hepatocyte growth factor G5 supplement Oligodendrocyte |
| ISSN号 | 0301-4681 |
| 通讯作者 | xingzhihu@gmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Generation of homogeneous oligodendrocytes as donor cells is essential for human embryonic stem cell (hESC)-based cell therapy for demylinating diseases. Herein we present a novel method for efficiently obtaining mature oligodendrocytes from hESCs with high purity (79.7 +/- 6.9%), using hepatocyte growth factor (HGF) and G5 supplement(containing insulin, transferrin, selenite, biotin, hydrocortisone, basic fibroblast growth factor and epidermal growth factor) in a four-step method. We induced hESCs into neural progenitors (NP) with HGF (5 ng/ml) and G5 (1 x) supplemented medium in an adherent differentiation system. The purified NPs were amplified in suspension as neurospheres for 1 month, and terminal oligodendrocyte differentiation was then induced by G5 supplement withdrawal and HGF treatment (20 ng/ml). The cells generated displayed typical morphologies of mature oligodendrocytes and expressed oligodendrocyte markers O4 and myelin basic protein (MBP). Our result revealed that HGF significantly enhanced the proliferation of hESC-derived NPs and promoted the differentiation as well as the maturation of oligodendrocytes from NPs. Further studies suggest that HGF/c-Met signaling pathway might play an important role in oligodendrocyte differentiation in our system. Our studies provide a means for generating the clinically relevant cell type and a platform for deciphering the molecular mechanisms that control oligodendrocyte differentiation. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by research grants from Yunnan Nature Science Foundation 2001C0009Z, Major State research Development Program G200016108, Chinese Academy of Sciences KSCX1-05 and Chinese National Science Foundation 30370166. We are grateful to Michael Scott Wood for editing the manuscript. |
| 原文出处 | 200978177.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5633]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Laboratory of Reproduction Development Biology, Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan 650223, China 3.Graduate School, Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Hua ZX[*],Li TQ,Zhang XZ,et al. Hepatocyte growth factor enhances the generation of high-purity oligodendrocytes from human embryonic stem cells[J]. DIFFERENTIATION,2009,78(37655):177-184. |
| APA | Hua ZX[*],Li TQ,Zhang XZ,&Chen YC.(2009).Hepatocyte growth factor enhances the generation of high-purity oligodendrocytes from human embryonic stem cells.DIFFERENTIATION,78(37655),177-184. |
| MLA | Hua ZX[*],et al."Hepatocyte growth factor enhances the generation of high-purity oligodendrocytes from human embryonic stem cells".DIFFERENTIATION 78.37655(2009):177-184. |
入库方式： OAI收割
来源：昆明动物研究所
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