Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium
文献类型:期刊论文
作者 | Ji SH1,3,4; Jin LF[*]1,2; Guo XY1,3,4; Ji WZ[*]1,3,5 |
刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL |
出版日期 | 2010 |
卷号 | 46期号:8页码:693-701 |
关键词 | Hepatic progenitor cells Bipotency Serum free Extracelullar matrix Rhesus monkey |
通讯作者 | lifangj@sohu.com ; wji@mail.kiz.ac.cn |
合作状况 | 其它 |
英文摘要 | Studies with hepatic progenitor cells from non-human primates would allow better understanding of their human counterparts. In this study, rhesus monkey liver epithelial progenitor cells (mLEPCs) were derived from a small piece of newborn livers in chemical defined serum-free medium. Digested hepatic cells were treated in Ca2+-containing medium to form cell aggregates. Two types of cell aggregates were generated: elongated spindle cells and polygonal epithelial cells. Elongated spindle cells were expressed as vimentin and brachyury, and they were disappeared within 5 d in our cultures. The remaining type consisted of small polygonal epithelial cells that expressed cytokeratin 7 (CK7), CK8, CK18, nestin, CD49f, and E-cad, the markers of hepatic stem cells, but were negative for alpha-fetoprotein, albumin, and CK19. They can proliferate and be passaged, if on laminin or rat tail collagen gel, to initiate colonies. When cultured with dexamethasone and oncostatin M, the expression of mature hepatocyte markers, such as alpha-1-antitrypsin, intracytoplasmic glycogen storage, indocyanine green uptake, and lipid droplet generation, were induced in differentiated cells. If transferred onto mouse embryonic fibroblasts feeders, they gave rise to CK19-positive cholangiocytes with formation of doughnut-like structure. Thus, mLEPCs with bipotency were derived from newborn monkey liver and may serve as a preclinical model for assessment of cell therapy in humans |
资助信息 | This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425. |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2010-11-01 |
源URL | [http://159.226.149.42:8088/handle/152453/6347] |
专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
作者单位 | 1.Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, Kunming, Yunnan 650223, China 2.College of Life Science, Shaoxing University, 900# Chennan Dadao, Shaoxing, Zhejiang 312000, China 3.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming, Yunnan, China 4.Graduate School, Chinese Academy of Sciences, Beijing, China 5.Kunming Biomed International, Kunming, Yunnan, China |
推荐引用方式 GB/T 7714 | Ji SH,Jin LF[*],Guo XY,et al. Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2010,46(8):693-701. |
APA | Ji SH,Jin LF[*],Guo XY,&Ji WZ[*].(2010).Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,46(8),693-701. |
MLA | Ji SH,et al."Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 46.8(2010):693-701. |
入库方式: OAI收割
来源:昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。