中缅树鼩精子形态特征及冷冻损伤
文献类型：期刊论文
| 作者 | 张远旭1,2; 平述煌1; 杨世华[*]1 |
| 刊名 | 动物学研究
 |
| 出版日期 | 2012 |
| 卷号 | 33期号:1页码:29−36 |
| 关键词 | 树鼩 睾丸 精子 形态结构 冷冻损伤 |
| 通讯作者 | huashiyang@hotmail.com |
| 合作状况 | 其它 |
| 中文摘要 | 树鼩作为一种新型的、接近灵长类的实验动物, 在医学生物学上的应用受到越来越多的重视。精子的结构特性研究及冷冻后结构的完整性分析是精子生物学的主要内容, 也有助于树鼩的实验室快速繁殖。该研究采用人工饲养的中缅树鼩(Tupaia belangeri chinensis), 结果显示其睾丸占总体重的(1.05 0.07)%, 总体积为(1.12±0.10) mL。附睾尾及输精管精子总量估计在2.2×107～8.8×107, 其运动度和顶体完整率分别为(68.8±3.9)%和(90.0±2.1)%。利用扫描电子显微镜和透射电子显微镜对树鼩附睾精子的超微结构进行的观察和分析显示精子头部呈圆形或卵圆形; 头部长度、宽度平均分别为6.65 和5.82 μm; 精子尾部中段、主段、尾段和精子总长度平均分别为13.39、52.35、65.74 和73.05 μm; 尾部中段的线粒体螺旋数量为48 个, 其轴丝结构为典型的“9+9+2”结构。冷冻解冻后的精子主要表现在顶体与质膜不完整、精子断裂、尾部扭曲和膨大。上述结果提示树鼩精子与其他哺乳动物精子的结构特征相似, 但是精子大小和线粒体螺旋数目有明显的差别, 且超微结构改变仍是冷冻精子运动 和受精能力下降的主要原因。 |
| 英文摘要 | The tree shrew (Tupaia belangeri chinensis) is a small non-rodent mammal, which is a relatively new experimental animal in medicine due to its close evolutionary relationship to primates and its rapid propagation. Sperm characteristics and cryopreservation in the tree shrew were the main contents of our spermatological research. Epididymal sperm were surgically harvested from male tree shrews captured from the Kunming area. The rate of testis weight to body weight was (1.05±0.07)%, volume of both testis was (1.12±0.10) mL, total sperm from epididymis and vas deferens were 2.2−8.8×107, and sperm motility and acrosome integrity were (68.8±3.9)% and (90.0±2.1)%, respectively. Sperm ultrastructure of the tree shrew was examined by scanning electron microscopy and transmission electron microscopy. Tree shrew sperm had a round or oval shaped head of approximately 6.65×5.82 μm, and midpiece, principal piece, tail, and total sperm lengths were 13.39, 52.35, 65.74, and 73.05 μm, respectively. The mitochondria in the midpiece consisted of approximately 48 gyres and had a 9+9+2 axonemal pattern. After freezing and thawing, sperm showed partly intact acrosomes and plasma membrane defects, and sperm breakages, twists, and swellings were found. The tree shrew had similar ultrastructure with other mammalians except for the mitochondria number and the sperm size. Ultrastructural alteration is still the main cause resulting in poor sperm after cryopreservation. |
| 收录类别 | 其他 |
| 资助信息 | 国家自然科学基金(31071279). |
| 语种 | 中文 |
| 公开日期 | 2013-06-18 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7490]  |
| 专题 | 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.昆明理工大学生命科学与技术学院衰老与肿瘤分子遗传学实验室, 云南 昆明 650500 2.中国科学院和云南省动物模型与人类疾病机理重点实验室, 中国科学院昆明动物研究所, 云南 昆明 650223 |
| 推荐引用方式 GB/T 7714 | 张远旭,平述煌,杨世华[*]. 中缅树鼩精子形态特征及冷冻损伤[J]. 动物学研究,2012,33(1):29−36. |
| APA | 张远旭,平述煌,&杨世华[*].(2012).中缅树鼩精子形态特征及冷冻损伤.动物学研究,33(1),29−36. |
| MLA | 张远旭,et al."中缅树鼩精子形态特征及冷冻损伤".动物学研究 33.1(2012):29−36. |
入库方式： OAI收割
来源：昆明动物研究所
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