Unravelling the proteome of adult rhesus monkey ovaries
文献类型：期刊论文
| 作者 | He H1; Teng H1; Zhou T1; Guo YS1; Wang GG1; Lin M1; Sun YJ1; Si W2; Zhou ZM1; Guo XJ[*]1,2 |
| 刊名 | MOLECULAR BIOSYSTEMS
 |
| 出版日期 | 2014 |
| 卷号 | 10期号:3页码:653-662 |
| 通讯作者 | guo_xuejiang@njmu.edu.cn ; huoran@njmu.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Ovarian physiology and pathology are important areas of scientific research. Efforts have been made to identify the ovary-related transcriptomes in different species. However, the proteomic studies are limited. The rhesus monkey is very similar to humans, and it is widely used in the study of reproductive biology and medicine. In this study, using an optimized proteomics platform, we successfully identified 5723 rhesus ovarian proteins, of which 4325 proteins were consistently identified in all three replicates and with at least 2 unique peptides. The 4325 proteins were chosen for further analysis. Through gene ontology and pathway analyses, we obtained a preliminary understanding of the function of these proteins. A random immunohistochemistry analysis was used to determine the expression of proteins in various cell types. By comparing the genes identified in this study with genes that were reported to have relatively high levels of expression in human oocytes, we obtained genes that were predicted to play roles in maintenance of normal ovarian physiology. Searching the identified genes from this study against the MGI database gave us a list of proteins those exist in the rhesus monkey ovary and are important for female mouse reproduction as well. The overlap of genes in this study and the genes whose abnormal expression or dysfunction were reported to be associated with human polycystic ovary syndrome (PCOS) and premature ovarian failure (POF) prompted us to use the rhesus monkey to study these two common causes of female infertility. This study may provide a basis for future studies of human reproductive disorders using the rhesus monkey as a model. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by grants from the 973 program (2012CB944704 and 2012CBA01306). |
| 语种 | 英语 |
| WOS记录号 | WOS:000330798200029 |
| 公开日期 | 2014-03-14 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7818]  |
| 专题 | 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, Jiangsu, China 2.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China |
| 推荐引用方式 GB/T 7714 | He H,Teng H,Zhou T,et al. Unravelling the proteome of adult rhesus monkey ovaries[J]. MOLECULAR BIOSYSTEMS,2014,10(3):653-662. |
| APA | He H.,Teng H.,Zhou T.,Guo YS.,Wang GG.,...&Huo R[*].(2014).Unravelling the proteome of adult rhesus monkey ovaries.MOLECULAR BIOSYSTEMS,10(3),653-662. |
| MLA | He H,et al."Unravelling the proteome of adult rhesus monkey ovaries".MOLECULAR BIOSYSTEMS 10.3(2014):653-662. |
入库方式： OAI收割
来源：昆明动物研究所
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