Cryopreservation of kunming mouse oocytes using slow cooling, ultrarapid cooling and vitrification protocols
文献类型：期刊论文
| 作者 | Men HS; Chen JC; Zou RJ; Ji WZ[*]; Shang EY; Yang SC |
| 刊名 | Theriogenology
 |
| 出版日期 | 1997 |
| 卷号 | 47期号:7页码:1423-1431 |
| 关键词 | Kunming mouse oocytes slow cooling ultrarapid cooling vitrification cryoprotectants |
| 英文摘要 | The cryopreservation of oocytes has been only marginally successful with any of the current protocols, including slow cooling, rapid cooling and vitrification. We wished to test the hypothesis that oocytes from a single mouse strain would freeze successfully by 1 of the 3 mentioned protocols. Unfertilized Kunming mouse oocytes obtained 14 h after PMSG/hCG administration were randomly assigned to be cryopreserved after slow cooling, ultra rapid cooling and vitrification. Oocytes were thawed by straws being placed into 37 degrees C water, and their morphological appearance and in vitro fertilization capability were compared with that of oocytes that had not undergone cryopreservation. Survival of oocytes was indicated by the absence of darkened ooplasm or by broken membranes or zona pellucida. Functional integrity was evaluated by the formation of a 2-cell embryo after IVF. Survival rate of slow cooled oocytes did not differ from that seen in vitrified oocytes (55.1 vs 65.9%) but was significantly lower in the rapidly cooled oocytes (24.2%; P<0.01). The results of NF of slow cooled and vitrified oocytes were similar to those of the control group (72 and 73 vs 77%; P>0.05). It appears that Kunming mouse oocytes can be successfully cryopreserved using the slow cooling method with 1,2-propanediol and vitrification, which contains both permeating and nonpermeating cryoprotectants. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by funds from the National Nature Sciences Foundation, fund No. 39170109, and from the Applied and Basic Research Fund of Yunnan |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9688]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_神经系统编码 |
| 作者单位 | Kunming Institute of Zoology, Kunming Cell Bank, The Chinese Academy of Sciences Kunming, Yunnan 650223, P.R. China |
| 推荐引用方式 GB/T 7714 | Men HS,Chen JC,Zou RJ,et al. Cryopreservation of kunming mouse oocytes using slow cooling, ultrarapid cooling and vitrification protocols[J]. Theriogenology,1997,47(7):1423-1431. |
| APA | Men HS,Chen JC,Zou RJ,Ji WZ[*],Shang EY,&Yang SC.(1997).Cryopreservation of kunming mouse oocytes using slow cooling, ultrarapid cooling and vitrification protocols.Theriogenology,47(7),1423-1431. |
| MLA | Men HS,et al."Cryopreservation of kunming mouse oocytes using slow cooling, ultrarapid cooling and vitrification protocols".Theriogenology 47.7(1997):1423-1431. |
入库方式： OAI收割
来源：昆明动物研究所
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