Correlation of zona-binding with oocyte maturation and sperm motility in rhesus monkeys by hemizona assay
文献类型：期刊论文
| 作者 | Pu XC; Ji WZ[*]; Shang EY; Yang SC; Chen JC; Zou RJ |
| 刊名 | MOLECULAR REPRODUCTION AND DEVELOPMENT
 |
| 出版日期 | 1994 |
| 卷号 | 39期号:1页码:25-29 |
| 关键词 | Hemizona assay (HZA) Rhesus monkey Oocyte Sperm |
| 英文摘要 | The hemizona assay (HZA) in Rhesus monkeys was employed to study the correlation of zona-binding ability with sperm motility or with naturally developing oocytes at various maturational stages. Oocytes from unstimulated ovaries were retrieved within 2 hr from monkeys sacrificed for vaccine production (in reproductive season, but with their menstrual cycles not determined). Oocytes were divided into four groups based on their morphological maturation: 1) Oocytes surrounded by more than one cumulus layer (MC); 2) Oocytes retaining intact germinal vesicle nuclei (GV); 3) Oocytes with germinal vesicle breakdown showing distinct perivitelline space (PVS); and 4) Oocytes extruding the first polar body (PB1). The mean numbers of sperm bound to hemizona for PB1, PVS, GV, and MC groups were 132.9 +/- 12.0, 71.5 +/- 10.1, 36.1 +/- 4.0, and 20.1 +/- 2.9 (Mean +/- SE), respectively. The four groups showed significant differences from each other in sperm/egg binding ability (P < 0.01). The number of bound sperm significantly increased with oocyte maturation. The present study also showed that zona-binding ability was also affected by sperm motility. For sperm with 67.7% motility and sperm with 31.2% motility, the average numbers of bound sperm were 43.5 +/- 2.2 and 25.3 +/- 2.9 (Mean +/- SE), respectively. There was significantly higher binding ability for sperm with higher motility (P < 0.01). The results suggest that: 1) The rhesus monkey model can serve as a very sensitive model for studying sperm/egg interaction by HZA; 2) Sperm motility positively correlated with sperm/egg binding; and 3) Sperm/egg binding ability increases with oocyte maturation. |
| 收录类别 | SCI |
| 资助信息 | This research was supported by Chinese National Nature and Science Grant 39170109, the Applied and Basic Research Fund of Yunnan Grant 93C066, and the Rockefeller Foundation on Biology of Reproduction through the Chinese Academy of Sciences. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9691]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_神经系统编码 |
| 作者单位 | Kunming Institute of Zoology, Academia Sinica, Kunming, China |
| 推荐引用方式 GB/T 7714 | Pu XC,Ji WZ[*],Shang EY,et al. Correlation of zona-binding with oocyte maturation and sperm motility in rhesus monkeys by hemizona assay[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,1994,39(1):25-29. |
| APA | Pu XC,Ji WZ[*],Shang EY,Yang SC,Chen JC,&Zou RJ.(1994).Correlation of zona-binding with oocyte maturation and sperm motility in rhesus monkeys by hemizona assay.MOLECULAR REPRODUCTION AND DEVELOPMENT,39(1),25-29. |
| MLA | Pu XC,et al."Correlation of zona-binding with oocyte maturation and sperm motility in rhesus monkeys by hemizona assay".MOLECULAR REPRODUCTION AND DEVELOPMENT 39.1(1994):25-29. |
入库方式： OAI收割
来源：昆明动物研究所
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