Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling
文献类型：期刊论文
| 作者 | Mitalipov SM[*]1; Zhou Q2; Wolf DP1; Byrne JA1; Ji WZ3; Norgren RB4 |
| 刊名 | HUMAN REPRODUCTION
 |
| 出版日期 | 2007 |
| 卷号 | 22期号:8页码:2232-2242 |
| 关键词 | somatic cell nuclear transfer nuclear remodeling premature cytoplast activation lamin A/C primate |
| 通讯作者 | mitalipo@ohsu.edu |
| 合作状况 | 其它 |
| 英文摘要 | BACKGROUND: Somatic cell nuclear transfer (SCNT) requires cytoplast-mediated reprogramming of the donor nucleus. Cytoplast factors such as maturation promoting factor are implicated based on their involvement in nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC). Given prior difficulties in SCNT in primates using conventional protocols, we hypothesized that the ability of cytoplasts to induce nuclear remodeling was instrumental in efficient reprogramming. METHODS: NEBD and PCC in monkey (Macaca mulatta) SCNT embryos were monitored by lamin A/C immunolabeling. RESULTS: Initially, a persistent lamin A/C signal from donor cell nuclei after fusion with cytoplasts was observed indicative of incomplete NEBD following SCNT and predictive of developmental arrest. We then identified fluorochrome-assisted enucleation and donor cell ellectrofusion as likely candidates for inducing premature cytoplast activation and a consequent lack of nuclear remodeling. Modified protocols designed to prevent premature cytoplast activation during SCNT showed robust NEBD and PCC. Coincidently, over 20% of SCNT embryos reconstructed with fetal fibroblasts progressed to blastocysts. Similar results were obtained with other somatic cells. Reconstructed blastocysts displayed patterns of Oct-4 expression similar to fertilized embryos reflecting successful reprogramming. CONCLUSIONS: Our results represent a significant breakthrough in elucidating the role of nuclear remodeling events in reprogramming following SCNT. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by NIH grants NS044330 and RR16030 to S. Mitalipov, HD18185 to R. Stouffer and RR00163 to D. Dorsa; the Chinese Academy of Sciences KSCX1-05, and Major State Research Development Program 2006CB701500. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10526]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA 2.Institute of Zoology, Chinese Academy of Sciences, Beijing, Chin 3.Kunming Institute of Zoology, Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan, China 4.Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, NE, USA |
| 推荐引用方式 GB/T 7714 | Mitalipov SM[*],Zhou Q,Wolf DP,et al. Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling[J]. HUMAN REPRODUCTION,2007,22(8):2232-2242. |
| APA | Mitalipov SM[*],Zhou Q,Wolf DP,Byrne JA,Ji WZ,&Norgren RB.(2007).Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling.HUMAN REPRODUCTION,22(8),2232-2242. |
| MLA | Mitalipov SM[*],et al."Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling".HUMAN REPRODUCTION 22.8(2007):2232-2242. |
入库方式： OAI收割
来源：昆明动物研究所
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