Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis
文献类型：期刊论文
| 作者 | Yu Y1,3; Ding CH1,2,3; Zhao CL1; Fan Y1; Wang L1; Beaujean N4; Zhou Q1; Jouneau A[*]1,4; Wang EY1,3; Ji WZ[*]2 |
| 刊名 | REPRODUCTION
 |
| 出版日期 | 2007 |
| 卷号 | 133期号:5页码:947-954 |
| 通讯作者 | alice.jouneau@jouy.inra.fr ; wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Even though it generates healthy adults, nuclear transfer in mammals remains an inefficient process. Mainly attributed to abnormal reprograming of the donor chromatin, this inefficiency may also be caused at least partly by a specific effect of the cloning technique which has not yet been well investigated. There are two main procedures for transferring nuclei into enucleated oocytes: fusion and piezoelectric microinjection, the latter being used mostly in mice. We have, therefore, decided to compare the quality and the developmental ability, both in vivo and in vitro, of embryos reconstructed with electrofusion or piezoelectric injection. In addition, the effect of piezo setups of differing electric strengths was investigated. Along with the record of the rate of development, we compared the nuclear integrity in the blastomeres during the first cleavages as well as the morphological and cellular quality of the blastocysts. Our results show that the piezo-assisted micromanipulation can induce DNA damage in the reconstructed embryos, apoptosis, and reduced cell numbers in blastocysts as well as a lower rate of development to term. Even if piezo-driven injection facilitates a faster and more efficient rate of reconstruction, it should be used with precaution and with as low parameters as possible. |
| 收录类别 | SCI |
| 资助信息 | Financial supports were from China National Basic Research Program 2006CB701501, PRA B 04-04 and The National Science Foundation of China 30300176 and 30525040. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10536]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 2.Department of Reproduction and Development, Kunming Institute of Zoologyand Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan, China 3.Graduate University of Chinese Academy of Sciences, Beijing, China 4.INRA, UMR 1198; ENVA; CNRS, FRE 2857, Biologie du De ´veloppement et Reproduction, Jouy en Josas 78350, France |
| 推荐引用方式 GB/T 7714 | Yu Y,Ding CH,Zhao CL,et al. Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis[J]. REPRODUCTION,2007,133(5):947-954. |
| APA | Yu Y.,Ding CH.,Zhao CL.,Fan Y.,Wang L.,...&Li XM.(2007).Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis.REPRODUCTION,133(5),947-954. |
| MLA | Yu Y,et al."Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis".REPRODUCTION 133.5(2007):947-954. |
入库方式： OAI收割
来源：昆明动物研究所
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