Expression of Hsp70-2 in Unilateral Cryptorchid Testis of Rhesus Monkey During Germ Cell Apoptosis
文献类型：期刊论文
| 作者 | Zhou XC1; Han XB1; Hu ZY1; Zou RJ2; Liu YX[*]1 |
| 刊名 | Endocrine
 |
| 出版日期 | 2001 |
| 卷号 | 16期号:2页码:89-95 |
| 关键词 | Apoptosis cryptorchid testis heat stress Hsp70-2 |
| 通讯作者 | liuyx@ panda.ioz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | We investigated the possible role of Hsp70-2 in germ cell apoptosis induced by heat stress in monkey unilateral cryptorchid testis. The study focused on in situ analysis of the testicular cell DNA fragmentation and on the possible relationship between Hsp70-2 expression and germ cell apoptosis. The TUNEL result showed that most of the germ cells were labeled in the cryptorchid testis on d 5 after induction of cryptorchidism; that with most of the apoptotic germ cells depleted, only a few germ cells were labeled on d 10; and that almost no apoptotic signal was observed in the cryptorchid testis on d IS and thereafter. This indicates that the increasing germ cell degeneration in cryptorchid testis may take the form of apoptosis. Using in situ hybridization, immunohistochemistry, and Northern blot, we examined the changes of Hsp70-2 expression in the monkey cryptorchid testis. The level of Hsp70-2 mRNA decreased slightly, while the expression of HSP70-2 protein was almost unchanged at the early stage of germ cell apoptosis in the cryptorchid testis on d 5 and dropped dramatically along with the loss of apoptotic germ cells in the cryptorchid testis on d 10 after operation. It is therefore suggested that Hsp70-2 might not take part in inhibiting the apoptosis of germ cells at the early stage during operation-induced cryptorchid testis, and that Hsp70-2 gene does not belong to the immediate early related gene responsible for germ cell apoptosis induced by heat stress. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by 973(G1997055901), Chuang-Xin, and Pandeng Programs, National Natural Science Founda- tion of China (39870368, 39670285), and the Chinese Acad- emy of Sciences (KJ982-J1-610). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10633]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China |
| 推荐引用方式 GB/T 7714 | Zhou XC,Han XB,Hu ZY,et al. Expression of Hsp70-2 in Unilateral Cryptorchid Testis of Rhesus Monkey During Germ Cell Apoptosis[J]. Endocrine,2001,16(2):89-95. |
| APA | Zhou XC,Han XB,Hu ZY,Zou RJ,&Liu YX[*].(2001).Expression of Hsp70-2 in Unilateral Cryptorchid Testis of Rhesus Monkey During Germ Cell Apoptosis.Endocrine,16(2),89-95. |
| MLA | Zhou XC,et al."Expression of Hsp70-2 in Unilateral Cryptorchid Testis of Rhesus Monkey During Germ Cell Apoptosis".Endocrine 16.2(2001):89-95. |
入库方式： OAI收割
来源：昆明动物研究所
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