Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
文献类型：期刊论文
| 作者 | Cai K1,2; Yang J1; Guan M1; Ji W1; Li Y1,3; Rens W4 |
| 刊名 | ARCHIVES OF ANDROLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 51期号:5页码:371-383 |
| 关键词 | flow cytometry fluorescent probes rhesus monkey spermatozoa viability |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 英文摘要 | Many fluorescent probes excited by visible light have been used to assess sperm quality by flow cytometry. Developing a viability evaluation method using UV excited stains would be useful for multiparameter analysis of sperm function. This investigation was conducted to determine the efficacy of Hoechst 33342 (H342) and propidium iodide (PI) dual staining for evaluating rhesus monkey sperm viability through use of flow cytometry and excited by a single UV laser. The results showed that the live cells stained only with H342 strongly correlated with expected sperm viability, and flow cytometric analyses were highly correlated with fluorescence microscopic observation. Using H342/PI/SYBR-14 triple staining method, it was found that the live/dead sperm distributions were completely concordant in both H342/PI and SYBR-14/PI assays. In addition, this dual staining was extended with fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA) to simultaneously analyze viability and acrosome integrity of sperm cryopreserved using two different extenders, TTE and TEST, and indicated that TTE offered better Preservation of plasma and acrosome integrity than TEST Therefore, the H342/PI dual staining provides an accurate technique for evaluating viability of rhesus monkey sperm and should be valuable for multiparameter flow cytometric analysis of sperm function. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the China National Science Foundation (30370166), Major Basic Research Program (G2000016108), and the Chinese Academy of Sciences (KSCX1-05). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10886]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming Yunnan, P.R.China 2.Graduate School, The Chinese Academy of Sciences, Beijing, P.R.China 3.College of Food Science and Technology, Yunnan Agricultural University, Kunming Yunnan, P.R.China 4.Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK |
| 推荐引用方式 GB/T 7714 | Cai K,Yang J,Guan M,et al. Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry[J]. ARCHIVES OF ANDROLOGY,2005,51(5):371-383. |
| APA | Cai K,Yang J,Guan M,Ji W,Li Y,&Rens W.(2005).Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry.ARCHIVES OF ANDROLOGY,51(5),371-383. |
| MLA | Cai K,et al."Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry".ARCHIVES OF ANDROLOGY 51.5(2005):371-383. |
入库方式： OAI收割
来源：昆明动物研究所
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