肝细胞生长因子促进猕猴胚胎干细胞来源的神经前体细胞的增殖
文献类型：学位论文
| 作者 | 李荣荣 |
| 学位类别 | 硕士 |
| 答辩日期 | 2008-06 |
| 授予单位 | 中国科学院研究生院 |
| 授予地点 | 北京 |
| 导师 | 季维智 |
| 关键词 | 猕猴胚胎干细胞 神经前体细胞 HGF |
| 其他题名 | Hepatocyte Growth Factor Promotes the Proliferation of the Neural Progenitors Derived from Rhesus Monkey Embryonic |
| 学位专业 | 细胞生物学 |
| 中文摘要 | 肝细胞生长因子（HGF）是一个多效应因子，在神经系统中具有重要作用，但是对于HGF在早期神经系统发育（特别是哺乳动物）中的具体作用还不明确，这方面的研究还很少。我们早前的研究发现采用HGF和G5 supplement结合EB法可诱导猕猴胚胎干细胞（rESCs）定向分化成高纯度（88.3± 8.1%）的可移植的神经前体细胞，但是HGF在整个分化过程中的具体作用及HGF与其它因子的关系还不清楚。 本研究改进先前研究体系，用单层培养诱导体系代替EB法诱导体系，用bFGF代替G5 supplement。即采用单层培养的方式，分别用同时含bFGF和HGF、只含HGF或bFGF以及两者都没有添加的成分确定的分化液诱导rESCs向神经细胞分化。并检测HGF对rESCs来源的神经前体细胞的增殖速度的影响，旨在进一步研究HGF在整个分化过程中的具体作用及HGF与bFGF的关系。主要结论如下：1）采用单层培养法，同时添加HGF和bFGF可诱导rESCs在两周内定向分化为高纯度（>85%）的神经前体细胞，从而建立了一种更为简单的诱导rESCs分化成神经细胞的方法；2）不同分化条件下都得到了相似比例的神经前体细胞，表明外源性的HGF在诱导rESC向神经前体细胞转变的过程中对于神经细胞命运的决定并不起作用；3）HGF能有效地促进rESCs来源的神经前体细胞的增殖，并且与bFGF具有协同作用。 |
| 英文摘要 | Hepatocyte growth factor (HGF) is a pleiotropic factor, playing important roles in the nervous system. However，the exact role of HGF in the process of early neural system development are not clear yet, especially in the mammal, the study about that lacks. We have previously produced highly enriched (88.3±8.1%) transplantable population of neural progenitor from rhesus monkey embryonic stem cells (rESCs) using an EB system containing HGF and G5 supplement. However, the exact role of HGF in the neural differentiation process as well as the relationshipt between HGF and other factors are not clear yet. Here, we ameliorated the previous differentiation system, sustituting adherent monoculture system for EB differentiation system and substituting bFGF for G5 supplement. The rESCs were inducted to differentiate toward neural cells in adherent monoculture system containing both HGF and bFGF, either HGF or bFGF alone or neither of them, and then the effete of HGF on Proliferation capability of the neural progenitors derived from rESCs was tested. Our stuy aimed at studying the exact role of HGF in the neural differentiation process as well as the relationship between HGF and bFGF. The results are shown as follows: 1. we efficiently produced population of neural progenitors (>85%) from rESCs in adherent monoculture system containing HGF and bFGF within two weeks，and a much simpler method of inducing rESCs differentiation towards neural cells has been established. 2. we got similar rates of the neural progenitors with the systems containing both HGF and bFGF, either HGF or bFGF alone, and even neither of them, which implied that extrinsic HGF can not determine the neural fate of the rESCs in the differentiation. 3. HGF with bFGF synergistically promoted the proliferation of the neural progenitors derived from rESCs. |
| 语种 | 中文 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6073]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 |
| 推荐引用方式 GB/T 7714 | 李荣荣. 肝细胞生长因子促进猕猴胚胎干细胞来源的神经前体细胞的增殖[D]. 北京. 中国科学院研究生院. 2008. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。