用RAPD技术对特化等级裂腹鱼类亲缘关系的探讨
文献类型：期刊论文
| 作者 | 陈自明1; 陈毅峰[*]2 |
| 刊名 | 动物学研究
 |
| 出版日期 | 2000 |
| 卷号 | 21期号:4页码:262-268 |
| 关键词 | RAPD 特化等级裂腹鱼类 亲缘关系 |
| ISSN号 | 0254-5853 |
| 中文摘要 | 用RAPD标记技术来分析属级或属级以上分类单元的亲缘关系,具有一定的适用性。对于一些遗传差异较大的类群(如裂腹鱼类),应用属级或属级以上分类单元的亲缘关系,很可能会出现个体之间遗传距离接近或达到100%的情况,从而无法探讨其相互之间的亲缘关系。 |
| 英文摘要 | The specialized schizothoracine fishes belong to subfamily Schizothoracinae (Cyprinidae).Compared to other fishes in the same subfamily,the specialized schizothoracine fishes have the following characters:1)only 1 pair of barbels,2)2 rows of pharyngeal teeth,3)scales degenerated,and even degenerated entirely in genus Gymnodiptychus (Cao et al.,1981).There are 9 species and subspecies belonging to 3 genera ［Diptychus (Steindachner,1866),Ptychobarbus (Steindachner,1866) and Gymnodiptychus (Herzenstein,1892)］ in this group.In this paper,random amplified polymorphic DNAs (RAPDs) were used to investigate genetic relationships among 22 individuals of the fishes,which represent 6 species and 4 genera,Schizothorax,Diptychus,Ptychobarbus and Gymnodiptychus respectively.234 RAPD markers were observed by using 37 arbitrary primers (10 bp).The molecular phylogenetic tree constructed by NJ (Neighbor-joining) method indicated that these 22 individuals could be divided into 6 clusters (each cluster represents one species).Upon the relationships among the 3 genera,Diptychus,Ptychobarbus and Gymnodiptychus,the conclusion was the same as that concluded from the phylogenetic analysis by using morphological characters.The result showed that RAPD could be used to study the phylogenetic relationships of closely related genera. |
| 收录类别 | 其他 |
| 资助信息 | 国家重大基础研究发展规划项目(G1998040800 );中国科学院重大项目A;中国科学院“九五”重点项目(KZ952-31-101) |
| 原文出处 | 2000214262.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/3751]  |
| 专题 | 昆明动物研究所_系统进化与生物地理学 |
| 作者单位 | 1.中国科学院昆明动物研究所,昆明650223 2.中国科学院水生生物研究所,武汉430072 |
| 推荐引用方式 GB/T 7714 | 陈自明,陈毅峰[*]. 用RAPD技术对特化等级裂腹鱼类亲缘关系的探讨[J]. 动物学研究,2000,21(4):262-268. |
| APA | 陈自明,&陈毅峰[*].(2000).用RAPD技术对特化等级裂腹鱼类亲缘关系的探讨.动物学研究,21(4),262-268. |
| MLA | 陈自明,et al."用RAPD技术对特化等级裂腹鱼类亲缘关系的探讨".动物学研究 21.4(2000):262-268. |
入库方式： OAI收割
来源：昆明动物研究所
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