丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响
文献类型：期刊论文
| 作者 | 谢玉波1; 刘敬臣1; 徐林[*]2; 曾邦雄3 |
| 刊名 | 临床麻醉学杂志
 |
| 出版日期 | 2005 |
| 卷号 | 21期号:6页码:399-401 |
| 关键词 | 兴奋性突触后电流 γ2氨基丁酸 受体 丙泊酚 海马 |
| 其他题名 | The effects of propofol on excitatory synaptic transmission in hippocampal CA1 neurons of rats |
| 合作状况 | 其它 |
| 中文摘要 | 目的观察500μmol/L丙泊酚对大鼠海马CA1区电刺激诱发的兴奋性突触后电流(EPSC)的影响,分析丙泊酚的可能作用机制。方法断头法分离Wistar大鼠(13~19d)海马半脑,用切片机切出400μm厚度的海马脑片,全细胞膜片钳技术记录CA1区锥体神经元EPSC。实验分两组:脂肪乳剂组(n=6)和丙泊酚组(n=10)。先以50μmol/L印防己毒素预孵脑片30min后,记录基础EPSC10min,然后加入450μl脂肪乳剂或丙泊酚(相当于500μmol/L),继续记录EPSC40min;继而以配对刺激代替单刺激,观察EPSC2/EPSC1比率的变化;改变膜钳制电压(-80~+60mV),观察电流-电压(I-V)曲线的变化。结果脂肪乳剂对EPSC无影响,500μmol/L丙泊酚降低大鼠海马CA1区EPSC值,25~30min左右达最大抑制效果,EPSC幅值下降至基础值的67·5%,明显低于脂肪乳剂组(P<0·05);而且500μmol/L丙泊酚明显降低EPSC2/EPSC1比率,也使I-V曲线左移,降低反转电位至-35mV左右。结论500μmol/L丙泊酚对大鼠海马CA1区兴奋性突触传递产生抑制作用,这可能与其增强突触前膜、突触后膜GABAA受体活性有关。 |
| 英文摘要 | Objective To investigate the effects of 500 μ mol/ L propofol on the whole 2 cell excit 2 atory postsynaptic current(EPSC) in hippocampal CA1 pyramidal neurons of rats and to analyze the mechanisms of propofol deeply. Methods Wistar rats(13 2 19 d) were killed by cervical dislocation and hippocampal slices (400 μ m) were prepared. EPSCs were recorded by whole 2 cell patch 2 clamp tech 2 nique. The experiments were divided into two groups: the intralipid group ( n = 6) and the propofol group( n = 10) . After hippocampal slices were preincubated with 50 μ mol/L picrotoxin for 30 min ,the base value was recorded for 10 min and then 450 μ l intralipid or propofol(500 μ mol/L) was applied in perfusion for another 40 min. The Schaffer collateral/ commissural pathway was stimulated with pair 2 pulses(interpulse interval was 50 ms) and the EPSC2/ EPSC1 ratio was analyzed. Moreover ,the hold 2 ing potential was changed from - 80 to + 60mV and the current 2 voltage curve was drawn. Results Intralipid didn ’ t affect EPSC ,but 500 μ mol/ L propofol reduced EPSC to (67 1 5 ± 1 1 7) % of the base value after application of propofol. 500 μ mol/ L propofol decreased the EPSC2/ EPSC1 ratio and shif 2 ted the current 2 voltage curve to the left. Conclusion 500 μ mol/ L propofol inhibits excitatory synaptic transmission in hippocampal CA1 pyramidal neurons of rats. The activity of GABA A receptor in the presynaptic and postsynaptic membrane may be increased with propofol applied. |
| 收录类别 | 其他 |
| 资助信息 | 本课题为国家自然科学基金资助项目(39930080) |
| 语种 | 中文 |
| 公开日期 | 2010-08-10 |
| 源URL | [http://159.226.149.42:8088/handle/152453/1141]  |
| 专题 | 昆明动物研究所_学习记忆的分子神经机制 |
| 作者单位 | 1.广西医科大学附属第一医院麻醉科,南宁530021 2.中国科学院昆明动物研究所学习与记忆实验室,昆明650223 3.华中科技大学同济医学院附属协和医院麻醉科 |
| 推荐引用方式 GB/T 7714 | 谢玉波,刘敬臣,徐林[*],等. 丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响[J]. 临床麻醉学杂志,2005,21(6):399-401. |
| APA | 谢玉波,刘敬臣,徐林[*],&曾邦雄.(2005).丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响.临床麻醉学杂志,21(6),399-401. |
| MLA | 谢玉波,et al."丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响".临床麻醉学杂志 21.6(2005):399-401. |
入库方式： OAI收割
来源：昆明动物研究所
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