Whole-scale neurobehavioral assessments of photothrombotic ischemia in freely moving mice
文献类型：期刊论文
| 作者 | Yu CL1,2; Zhou H1,2; Chai AP2; Yang YX2; Mao RR[*]2; Xu L[*]1,2 |
| 刊名 | JOURNAL OF NEUROSCIENCE METHODS
 |
| 出版日期 | 2015 |
| 卷号 | 239期号:X页码:100-107 |
| 关键词 | Freely moving mice Neurobehavioral assessments Nimodipine Photothrombotic ischemia |
| 通讯作者 | talktomaomao@163.com ; lxu@vip.163.com |
| 合作状况 | 其它 |
| 英文摘要 | Background: Neurobehavioral assessments have been considered as an essential component of preclinical research in ischemic stroke. However, real-time neurobehavioral evaluation is seldom applied during ischemia induction as it is usually accompanied with anesthesia. New method: We induced photothrombosis in freely moving mice after one-week recovery from cannula implantation surgeries. After rose bengal (RB) injection (100 mg/kg, i.p.), photothrombosis was induced in freely moving mice by 473 nm laser irradiation through the cannulas implanted into unilateral primary motor cortex beforehand. Mice received nimodipine (15 mg/kg, i.p.), a widely used anti-ischemic agent, or vehicle before irradiation. Motor coordination and equilibrium were evaluated by rotarod and rung walk tests throughout the whole process of ischemia. Endurance capacity was assessed by treadmill at 1 day and 7 days after irradiation. Mice were decapitated at different time points post irradiation for TTC (2,3,5-triphenyltetrazolium chloride) staining. Results: Consistent with the results of TTC staining, motor deficits firstly occurred at 15-min post irradiation and aggravated 1-day later, while the capacity improved 3-days later and partially recovered 7-days post irradiation. And, the recovery process was accelerated by nimodipine application. Comparison with existing methods: This method established a precise linkage between focal brain ischemia development and neurobehavioral deficits throughout a full scale of photothrombosis, which avoided the confounding factors of anesthetics and surgeries on neurobehavioral assessments, as infarct was induced in freely moving mice. Conclusions: This method with high temporal and spatial resolution will be an optimal model for neurobehavioral evaluation in preclinical anti-ischemic drug screening. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by National Basic Research Program of China (2013CB835103), NSFC-CIHR Joint Grant (81161120536), and Strategic Priority Research Program of the Chinese Academy of Science (XDB02020200), National Natural Science Foundation of China (31100786, 81171294, U1032605 and U1132602), Science and Technology Program of Yunnan Province (2013GA003 and 2010CD104). |
| 语种 | 英语 |
| 公开日期 | 2015-02-13 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8256]  |
| 专题 | 昆明动物研究所_学习记忆的分子神经机制 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.School of Life Sciences, University of Science and Technology of China, Hefei 230027, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms, and KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Disease, and Laboratory of Learning and Memory, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Yu CL,Zhou H,Chai AP,et al. Whole-scale neurobehavioral assessments of photothrombotic ischemia in freely moving mice[J]. JOURNAL OF NEUROSCIENCE METHODS,2015,239(X):100-107. |
| APA | Yu CL,Zhou H,Chai AP,Yang YX,Mao RR[*],&Xu L[*].(2015).Whole-scale neurobehavioral assessments of photothrombotic ischemia in freely moving mice.JOURNAL OF NEUROSCIENCE METHODS,239(X),100-107. |
| MLA | Yu CL,et al."Whole-scale neurobehavioral assessments of photothrombotic ischemia in freely moving mice".JOURNAL OF NEUROSCIENCE METHODS 239.X(2015):100-107. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。