Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain
文献类型：期刊论文
| 作者 | Guo J[*]1; Chen YH2; Zhou KY1; Li JY3 |
| 刊名 | BIOTECHNIC & HISTOCHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 80期号:1页码:31-34 |
| 关键词 | Giardia lamblia NOR nucleolus organizing region silver staining |
| ISSN号 | 0884-6812 |
| 通讯作者 | jguo58@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | Previous investigations have proved that diplomonads have primitive cell nuclei and lack a nucleolus. We determined the distribution of ribosomal DNA (rDNA) in diplomonad nuclei that lacked a nucleolus. Giardia lamblia was used as the experimental organism with Euglena gracilis as the control. The distribution of rDNA was demonstrated indirectly by the modified Ag-I silver technique that can indicate specifically the nucleolus organizing region (NOR) by both light and electron microscopy. In ultrathin sections of silver stained Euglena cells, all silver grains were concentrated in the fibrosa of the nucleolus, while no silver grains were found in the cytoplasm, nucleoplasm, condensed chromosomes or pars granulosa of the nucleus. In the silver stained Giardia cells, no nucleolus was found, but a few silver grains were scattered in the nucleus. This suggests that the rDNA of Giardia does not form an NOR-like structure and that its nucleus is in a primitive state. |
| 收录类别 | SCI |
| 资助信息 | This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094. |
| 原文出处 | 20052779.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2479]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 1.College of Life Science, Nanjing Normal University, Nanjing, 210097, P.R. China 2.Department of Histology and Embryology, Nanjing Military Medical College of the Second Military Medical University, Nanjing 3.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P.R. China |
| 推荐引用方式 GB/T 7714 | Guo J[*],Chen YH,Zhou KY,et al. Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain[J]. BIOTECHNIC & HISTOCHEMISTRY,2005,80(1):31-34. |
| APA | Guo J[*],Chen YH,Zhou KY,&Li JY.(2005).Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain.BIOTECHNIC & HISTOCHEMISTRY,80(1),31-34. |
| MLA | Guo J[*],et al."Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain".BIOTECHNIC & HISTOCHEMISTRY 80.1(2005):31-34. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。