源真核生物蓝氏贾第虫核分裂的初步观察
文献类型：期刊论文
| 作者 | 沈剑钊1; 李靖炎2; 卢思奇1 |
| 刊名 | 动物学研究
 |
| 出版日期 | 1996 |
| 卷号 | 17期号:3页码:295-299 |
| 关键词 | 贾第虫 核分裂 纺锤体 微管蛋白 秋水仙素 |
| ISSN号 | 0254-5853 |
| 其他题名 | The Preliminary Study on The Nuclear Division of The Archezoan,Giardia lamblia |
| 中文摘要 | 首次对蓝氏贾第虫(Giardlia lamblia)的核分裂作了电镜观察, 未能在分裂着的核中见到纺缍体或纺缍体微管。以秋水仙素实验和微管蛋白作免疫荧光检查后, 似乎意味着其核分裂方式乃是前有丝分裂性质的, 并作了讨论。 |
| 英文摘要 | Diplomonads seem to be the most primitive archezoa.In literature,almost all the studies on diplomonad nuclear division were accomplished under light microscope,Considerring the primitiveness of diplomonad nucleus,we investigated the nuclear division of cultivated Giardia lamblia with routine electron-microscopy preliminarily.Nuclear envelope was found to be preseved during the whole division process.Spindle or spindle microtubules were never found within dividing nucleus or cytoplasm.No condensed metaphase chromosomes were observed,although heterochromatin could occationally be seen both in dividing nucleus and in interphase nucleus.If there is surely no spinde participating the division process,then colchcine should have no influence on this process.Our experiments showed that various concentrations of colchicine (0.1-20 μg/ml),certainly had no effect on the cell division of Giardia.Immuno-fluorescent microscopical detection was done with anti-tubulin antibody and it was found that no tubulin or tubulin-made structure existed in the interphase nucleus and dividing nucleus,while cytoplasm and flagella gave strong positive reaction.The resuts described above suggested that there is no spindle formed during the nuclear division of Giardia.The possible significance of this phenomenon in the origin and evolution of mitosis was discussed. |
| 收录类别 | 其他 |
| 资助信息 | 国家自然科学基金 |
| 原文出处 | 1996173295.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/3451]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 1.首都医科大学寄生虫学教研室 2.中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223 |
| 推荐引用方式 GB/T 7714 | 沈剑钊,李靖炎,卢思奇. 源真核生物蓝氏贾第虫核分裂的初步观察[J]. 动物学研究,1996,17(3):295-299. |
| APA | 沈剑钊,李靖炎,&卢思奇.(1996).源真核生物蓝氏贾第虫核分裂的初步观察.动物学研究,17(3),295-299. |
| MLA | 沈剑钊,et al."源真核生物蓝氏贾第虫核分裂的初步观察".动物学研究 17.3(1996):295-299. |
入库方式： OAI收割
来源：昆明动物研究所
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