尖尾藻的染色质碱性蛋白研究
文献类型：期刊论文
| 作者 | 文建凡; 李靖炎 |
| 刊名 | 动物学报
 |
| 出版日期 | 1998 |
| 卷号 | 44期号:3页码:347-352 |
| 关键词 | 涡鞭毛虫 尖尾藻 染色质碱性蛋白 进化 |
| ISSN号 | 0001-7302 |
| 其他题名 | STUDIES ON BASIC CHROMATIN PROTEINS OF DINOFLAGELLATE OXYRRHIS MARINA |
| 合作状况 | 其它 |
| 中文摘要 | 利用“先固定后抽提”的方法，从尖尾藻细胞中提取出染色质碱性蛋白。经ＳＤＳ ＰＡＧＥ分析，结果显示此染色质碱性蛋白含有八个组分（记为１′、１″、２ａ、２ｂ、３′、３″、４′、４″），它们像小牛胸腺组蛋白一样形成两个分子量区域，即一为对应组蛋白Ｈ１的分子量区域，另一为对应于核心组蛋白（即Ｈ２Ａ，Ｈ２Ｂ，Ｈ３，Ｈ４）的分子量区域；其与组蛋白各组分的具体对应关系大致为：对应于小牛胸腺组蛋白Ｈ１两亚带区域有两条带１′、１″，对应于Ｈ３附近有两条带３′、３″，对应于Ｈ２Ａ、Ｈ２Ｂ分别有２ａ、２ｂ两带，对应于Ｈ４也有两条带４′、４″。激光密度扫描结果也清楚地显示出上述这些电泳带所对应的扫描峰。文中讨论了涡鞭毛虫类的进化地位。 |
| 英文摘要 | In most of the previous work , basic chromatin proteins (BCP) were isolated from di 2 noflagellates by the method which was invented for isolating histones from higher eukary 2 otes. But we have recently proved that this conventional method is not so suitable for the lower eukaryotes. In fact , the previous contradictory reports of BCP in dinoflagellates have reflected such a problem to a certain extent. For example , two reports showed that dinoflag 2 ellate Oxyrrhis marina had 1 and 2 BCP (s) , respectively. We have established a procedure called “ prefixation and acid extraction ” which avoids the above problem completely due to the methanol prefixation step before extraction of BCPs. By this procedure , BCPs were isolated from O. marina. SDS 2 PAGE analysis showed they contained 8 fractions , which had similar electrophoretic patterns with the five histone fractionsof calf thymus. Considering the advantage of our procedure over the conven 2 tional method , this result should be more reliable than the previous one. Most of the previous work had reported that dinoflagellates did not possess histone but contained 1 ～ 2 basic chromatin proteins , and some researchers thought the latter might be the ancestor of histones. According to the present work , in addition to our other work , we argue that BCPs of dinoflagellates are of diversity , and some dinoflagellates have much more than 1 ～ 2 BCPs which are similar to histones or are histones. The result support the opinion that “ the histones were secondarily lost in dinoflagellate ” and were replaced gradually by other basic proteins. So dinoflagellates are not as primitive as what they were thought be 2 fore , and the similarity of their chromosomes to nucleoid of bacteria might be a secondary revolutionary event after the loss of histones. |
| 收录类别 | 其他 |
| 原文出处 | 1998443347.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4751]  |
| 专题 | 昆明动物研究所_真核细胞进化基因组 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 中国科学院昆明动物研究所细胞与分子进化开放实验室,昆明650223 |
| 推荐引用方式 GB/T 7714 | 文建凡,李靖炎. 尖尾藻的染色质碱性蛋白研究[J]. 动物学报,1998,44(3):347-352. |
| APA | 文建凡,&李靖炎.(1998).尖尾藻的染色质碱性蛋白研究.动物学报,44(3),347-352. |
| MLA | 文建凡,et al."尖尾藻的染色质碱性蛋白研究".动物学报 44.3(1998):347-352. |
入库方式： OAI收割
来源：昆明动物研究所
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