The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?
文献类型：期刊论文
| 作者 | Wen JF[*] |
| 刊名 | BIOLOGY OF THE CELL
 |
| 出版日期 | 2000 |
| 卷号 | 92期号:2页码:125-131 |
| 关键词 | Euglena gracilis evolution of nuclear matrix evolution of lamins DGD-embedment free electron microscopy Western blot |
| ISSN号 | 0248-4900 |
| 通讯作者 | wenjf@mail.kiz.ac.cn |
| 英文摘要 | Euglena gracilis cell was extracted sequentially with CSK-Triton buffer, RSB-Magik solution and DNase-As solution. DGD embedment-free electron microscopy showed that in the extracted nucleus there was a residual non-chromatin fibrous network. That it could not be removed by hot trichloroacetic acid further supported the idea that it was a non-histone, non-chromatin fibrous protein network, and should be the internal network of the nuclear matrix. After the sequential extraction, the nuclear membrane was removed, leaving behind a layer of lamina; the chromatin was digested and eluted from the dense chromosomes and residual chromosomal structures that should be chromosomal scaffold were revealed. Western blot analysis with antiserum against rat lamins showed that nuclear lamina of the cell possessed two positive polypeptides, a major one and a minor one, which had molecular masses similar to lamin B and lamin A, respectively. Comparing these data with those of the most primitive eukaryote Archezoa and of higher eukaryotes, it was suggested that the lower unicellular eukaryote E. gracilis already had the nuclear matrix structure, and its nuclear matrix (especially the lamina) might represent a stage of evolutionary history of the nuclear matrix. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06). |
| 原文出处 | 200092125.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5947]  |
| 专题 | 昆明动物研究所_真核细胞进化基因组 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P.R. Chin |
| 推荐引用方式 GB/T 7714 | Wen JF[*]. The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?[J]. BIOLOGY OF THE CELL,2000,92(2):125-131. |
| APA | Wen JF[*].(2000).The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?.BIOLOGY OF THE CELL,92(2),125-131. |
| MLA | Wen JF[*]."The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?".BIOLOGY OF THE CELL 92.2(2000):125-131. |
入库方式： OAI收割
来源：昆明动物研究所
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