Identification, characteristic and phylogenetic analysis of type II DNA topoisomerase gene in Giardia lamblia
文献类型：期刊论文
| 作者 | He D1,2,3; Wen JF[*]1; Chen WQ1; Lu SQ4; Xin DD1,2 |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 15期号:6页码:474-482 |
| ISSN号 | 1001-0602 |
| 关键词 | evolutionary position GlTop2 gene Giardia lamblia identification phylogenetic analysis type II DNA topoisomerase. |
| 通讯作者 | wenjf@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The genes encoding type II DNA topoisomerases were investigated in Giardia lamblia genome, and a type IIA gene, GlTop 2 was identified. It is a single copy gene with a 4476 bp long ORF without intron. The deduced amino acid sequence shows strong homology to eukaryotic DNA Top 2. However, some distortions were found, such as six insertions in the ATPase domain and the central domain, a approximately 100 aa longer central domain; a approximately 200 aa shorter C-terminal domain containing rich charged residues. These features revealed by comparing with Top 2 of the host, human, might be helpful in exploiting drug selectivity for antigiardial therapy. Phylogenetic analysis of eukaryotic enzymes showed that kinetoplastids, plants, fungi, and animals were monophyletic groups, and the animal and fungi lineages shared a more recent common ancestor than either did with the plant lineage; microsporidia grouped with fungi. However, unlike many previous phylogenetic analyses, the "amitochondriate"G. lamblia was not the earliest branch but diverged after mitochondriate kinetoplastids in our trees. Both the finding of typical eukaryotic type IIA topoisomerase and the phylogenetic analysis suggest G. lamblia is not possibly as primitive as was regarded before and might diverge after the acquisition of mitochondria. This is consistent with the recent discovery of mitochondrial remnant organelles in G. lamblia. |
| 资助信息 | This work was supported by Grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China and Grants (KSCX2-SW-101C, STZ-00-23) from the Chinese Academy of Sciences. |
| 收录类别 | SCI |
| 原文出处 | 200515474.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5961]  |
| 专题 | 昆明动物研究所_真核细胞进化基因组 昆明动物研究所_细胞与分子进化重点实验室 |
| 作者单位 | 1.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Gradulate School of the Chinese Academy of Sciences, Beijing 100039, China 3.Southwest Forestry College, Kunming 650224, China 4.Capital University of Medical Sciences, Beijing 100054, China |
| 推荐引用方式 GB/T 7714 | He D,Wen JF[*],Chen WQ,et al. Identification, characteristic and phylogenetic analysis of type II DNA topoisomerase gene in Giardia lamblia[J]. CELL RESEARCH,2005,15(6):474-482. |
| APA | He D,Wen JF[*],Chen WQ,Lu SQ,&Xin DD.(2005).Identification, characteristic and phylogenetic analysis of type II DNA topoisomerase gene in Giardia lamblia.CELL RESEARCH,15(6),474-482. |
| MLA | He D,et al."Identification, characteristic and phylogenetic analysis of type II DNA topoisomerase gene in Giardia lamblia".CELL RESEARCH 15.6(2005):474-482. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。