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Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation

文献类型：期刊论文

作者Gao S1; Xiong J2,3; Zhang CC4; Yang RD6; Zhao M6; Molascon AJ1; Kwiatkowski SY1; Yuan DX2; Qin ZH6,7; Wen JF3
刊名GENES & DEVELOPMENT
出版日期2013
卷号27期号:15页码:1662-1679
关键词histone methyltransferase H3 Lys 27 methylation replication stress replication elongation ssDNA replication origin
通讯作者miaowei@ihb.ac.cn ; yifan@med.umich.edu
合作状况其它
英文摘要Replication of nuclear DNA occurs in the context of chromatin and is influenced by histone modifications. In the ciliate Tetrahymena thermophila, we identified TXR1, encoding a histone methyltransferase. TXR1 deletion resulted in severe DNA replication stress, manifested by the accumulation of ssDNA, production of aberrant replication intermediates, and activation of robust DNA damage responses. Paired-end Illumina sequencing of ssDNA revealed intergenic regions, including replication origins, as hot spots for replication stress in Delta TXR1 cells. Delta TXR1 cells showed a deficiency in histone H3 Lys 27 monomethylation (H3K27me1), while Delta EZL2 cells, deleting a Drosophila E(z) homolog, were deficient in H3K27 di- and trimethylation, with no detectable replication stress. A point mutation in histone H3 at Lys 27 (H3 K27Q) mirrored the phenotype of Delta TXR1, corroborating H3K27me1 as a key player in DNA replication. Additionally, we demonstrated interactions between TXR1 and proliferating cell nuclear antigen (PCNA). These findings support a conserved pathway through which H3K27me1 facilitates replication elongation.
收录类别SCI
资助信息This work was supported by NIH R01-GM087343 to Y.L., National Natural Science Founda- tion of China (31071993) and Fund of State Key Laboratory of Genetics Resources and Evolution (GREKF10-09) to W.M., NIH 1P41RR018627 to P.C.A., NIH R01-GM53572 to G.M.K., NIH R01-HG005119 to Z.Q., and University of Michigan Rackham Graduate Student Research Grant to C.Z.
语种英语
WOS记录号WOS:000322716000005
公开日期2013-09-13
源URL[http://159.226.149.42:8088/handle/152453/7627]  
专题昆明动物研究所_真核细胞进化基因组
昆明动物研究所_遗传资源与进化国家重点实验室
作者单位1.Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA
2.Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, China
4.Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, Michigan 48109, USA
5.Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M Health Science Center, College Station, Texas 77843, USA
6.Department of Biostatistics and Bioinformatics, Emory University, Atlanta, Georgia 30322, USA
7.Center for Comprehensive Informatics, Emory University, Atlanta, Georgia 30322, USA
8.Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
9.Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
推荐引用方式
GB/T 7714		 Gao S,Xiong J,Zhang CC,et al. Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation[J]. GENES & DEVELOPMENT,2013,27(15):1662-1679.
APA Gao S.,Xiong J.,Zhang CC.,Yang RD.,Zhao M.,...&Liu YF[*].(2013).Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation.GENES & DEVELOPMENT,27(15),1662-1679.
MLA Gao S,et al."Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation".GENES & DEVELOPMENT 27.15(2013):1662-1679.

入库方式： OAI收割

来源：昆明动物研究所

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