Deep Sequencing Reveals Transcriptome Re-Programming of Taxus x media Cells to the Elicitation with Methyl Jasmonate
文献类型：期刊论文
| 作者 | Sun GL1; Yang YF2; Xie FL3; Wen JF4; Wu JQ1; Wilson IW5; Tang Q6; Liu HW2; Qiu DY[*]2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:4页码:e62865 |
| 通讯作者 | qiudy@caf.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Background: Plant cell culture represents an alternative source for producing high-value secondary metabolites including paclitaxel (Taxol (R)), which is mainly produced in Taxus and has been widely used in cancer chemotherapy. The phytohormone methyl jasmonate (MeJA) can significantly increase the production of paclitaxel, which is induced in plants as a secondary metabolite possibly in defense against herbivores and pathogens. In cell culture, MeJA also elicits the accumulation of paclitaxel; however, the mechanism is still largely unknown. Methodology/Principal Findings: To obtain insight into the global regulation mechanism of MeJA in the steady state of paclitaxel production (7 days after MeJA addition), especially on paclitaxel biosynthesis, we sequenced the transcriptomes of MeJA-treated and untreated Taxus x media cells and obtained similar to 32.5 M high quality reads, from which 40,348 unique sequences were obtained by de novo assembly. Expression level analysis indicated that a large number of genes were associated with transcriptional regulation, DNA and histone modification, and MeJA signaling network. All the 29 known genes involved in the biosynthesis of terpenoid backbone and paclitaxel were found with 18 genes showing increased transcript abundance following elicitation of MeJA. The significantly up-regulated changes of 9 genes in paclitaxel biosynthesis were validated by qRT-PCR assays. According to the expression changes and the previously proposed enzyme functions, multiple candidates for the unknown steps in paclitaxel biosynthesis were identified. We also found some genes putatively involved in the transport and degradation of paclitaxel. Potential target prediction of miRNAs indicated that miRNAs may play an important role in the gene expression regulation following the elicitation of MeJA. Conclusions/Significance: Our results shed new light on the global regulation mechanism by which MeJA regulates the physiology of Taxus cells and is helpful to understand how MeJA elicits other plant species besides Taxus. |
| 收录类别 | SCI |
| 资助信息 | This work is supported by a grant from the National Natural Science Foundation of China (31170628) and a startup grant from Kunming Institute of Botany, Chinese Academy of Sciences, to the Group of Molecular Ecology, a grant from Yunnan Recruitment Program of Experts in Sciences (2012HA016), and a grant from Thousand Youth Talents Program. |
| 语种 | 英语 |
| WOS记录号 | WOS:000319077300105 |
| 公开日期 | 2014-10-17 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8087]  |
| 专题 | 昆明动物研究所_真核细胞进化基因组 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China 2.State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China 3.Department of Biology, East Carolina University, Greenville, North Carolina, United States of America 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 5.CSIRO Plant Industry, Canberra, Australian Capital Territory, Australia 6.Guangxi Botanical Garden of Medicinal Plant, Nanning, Guangxi, China |
| 推荐引用方式 GB/T 7714 | Sun GL,Yang YF,Xie FL,et al. Deep Sequencing Reveals Transcriptome Re-Programming of Taxus x media Cells to the Elicitation with Methyl Jasmonate[J]. PLOS ONE,2013,8(4):e62865. |
| APA | Sun GL.,Yang YF.,Xie FL.,Wen JF.,Wu JQ.,...&Qiu DY[*].(2013).Deep Sequencing Reveals Transcriptome Re-Programming of Taxus x media Cells to the Elicitation with Methyl Jasmonate.PLOS ONE,8(4),e62865. |
| MLA | Sun GL,et al."Deep Sequencing Reveals Transcriptome Re-Programming of Taxus x media Cells to the Elicitation with Methyl Jasmonate".PLOS ONE 8.4(2013):e62865. |
入库方式： OAI收割
来源：昆明动物研究所
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