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Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans

文献类型:期刊论文

作者Zhang YR1; Zou XJ2; Ding YH1; Wang HZ1; Wu XY1; Liang B[*]1
刊名BMC GENOMICS
出版日期2013
卷号14期号:X页码:e164
关键词Caenorhabditis elegans Lipid metabolism Comparative genomics RNAi Fat storage
通讯作者liangb@mail.kiz.ac.cn
英文摘要Background: Animal models are indispensable to understand the lipid metabolism and lipid metabolic diseases. Over the last decade, the nematode Caenorhabditis elegans has become a popular animal model for exploring the regulation of lipid metabolism, obesity, and obese-related diseases. However, the genomic and functional conservation of lipid metabolism from C. elegans to humans remains unknown. In the present study, we systematically analyzed genes involved in lipid metabolism in the C. elegans genome using comparative genomics. 

Results: We built a database containing 471 lipid genes from the C. elegans genome, and then assigned most of lipid genes into 16 different lipid metabolic pathways that were integrated into a network. Over 70% of C. elegans lipid genes have human orthologs, with 237 of 471 C. elegans lipid genes being conserved in humans, mice, rats, and Drosophila, of which 71 genes are specifically related to human metabolic diseases. Moreover, RNA-mediated interference (RNAi) was used to disrupt the expression of 356 of 471 lipid genes with available RNAi clones. We found that 21 genes strongly affect fat storage, development, reproduction, and other visible phenotypes, 6 of which have not previously been implicated in the regulation of fat metabolism and other phenotypes. 

Conclusions: This study provides the first systematic genomic insight into lipid metabolism in C. elegans, supporting the use of C. elegans as an increasingly prominent model in the study of metabolic diseases.
收录类别SCI
资助信息This work was supported by Chinese Natural Science Foundation to BL (31171134 and U1202223) and to XJ Zou (31160216), as well as Yunnan Natural Science Foundation to XJ Zou (2011FZ179).
语种英语
WOS记录号WOS:000316403000002
公开日期2013-04-11
源URL[http://159.226.149.42:8088/handle/152453/7355]  
专题昆明动物研究所_脂类代谢与疾病
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiao-Chang Dong Road, Kunming, Yunnan 650223, China
2.Department of Life Science and Biotechnology, Kunming University, Kunming 650214, China
推荐引用方式
GB/T 7714
Zhang YR,Zou XJ,Ding YH,et al. Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans[J]. BMC GENOMICS,2013,14(X):e164.
APA Zhang YR,Zou XJ,Ding YH,Wang HZ,Wu XY,&Liang B[*].(2013).Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans.BMC GENOMICS,14(X),e164.
MLA Zhang YR,et al."Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans".BMC GENOMICS 14.X(2013):e164.

入库方式: OAI收割

来源:昆明动物研究所

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