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Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans
文献类型：期刊论文
| 作者 | Zhang YR1; Zou XJ2; Ding YH1; Wang HZ1; Wu XY1; Liang B[*]1 |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2013 |
| 卷号 | 14期号:X页码:e164 |
| 关键词 | Caenorhabditis elegans Lipid metabolism Comparative genomics RNAi Fat storage |
| 通讯作者 | liangb@mail.kiz.ac.cn |
| 英文摘要 | Background: Animal models are indispensable to understand the lipid metabolism and lipid metabolic diseases. Over the last decade, the nematode Caenorhabditis elegans has become a popular animal model for exploring the regulation of lipid metabolism, obesity, and obese-related diseases. However, the genomic and functional conservation of lipid metabolism from C. elegans to humans remains unknown. In the present study, we systematically analyzed genes involved in lipid metabolism in the C. elegans genome using comparative genomics. Results: We built a database containing 471 lipid genes from the C. elegans genome, and then assigned most of lipid genes into 16 different lipid metabolic pathways that were integrated into a network. Over 70% of C. elegans lipid genes have human orthologs, with 237 of 471 C. elegans lipid genes being conserved in humans, mice, rats, and Drosophila, of which 71 genes are specifically related to human metabolic diseases. Moreover, RNA-mediated interference (RNAi) was used to disrupt the expression of 356 of 471 lipid genes with available RNAi clones. We found that 21 genes strongly affect fat storage, development, reproduction, and other visible phenotypes, 6 of which have not previously been implicated in the regulation of fat metabolism and other phenotypes. Conclusions: This study provides the first systematic genomic insight into lipid metabolism in C. elegans, supporting the use of C. elegans as an increasingly prominent model in the study of metabolic diseases. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Chinese Natural Science Foundation to BL (31171134 and U1202223) and to XJ Zou (31160216), as well as Yunnan Natural Science Foundation to XJ Zou (2011FZ179). |
| 语种 | 英语 |
| WOS记录号 | WOS:000316403000002 |
| 公开日期 | 2013-04-11 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7355]  |
| 专题 | 昆明动物研究所_脂类代谢与疾病 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiao-Chang Dong Road, Kunming, Yunnan 650223, China 2.Department of Life Science and Biotechnology, Kunming University, Kunming 650214, China |
| 推荐引用方式 GB/T 7714 | Zhang YR,Zou XJ,Ding YH,et al. Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans[J]. BMC GENOMICS,2013,14(X):e164. |
| APA | Zhang YR,Zou XJ,Ding YH,Wang HZ,Wu XY,&Liang B[*].(2013).Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans.BMC GENOMICS,14(X),e164. |
| MLA | Zhang YR,et al."Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans".BMC GENOMICS 14.X(2013):e164. |
入库方式： OAI收割
来源：昆明动物研究所
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