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Regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase
文献类型：期刊论文
| 作者 | Shi X1; Li J2,3; Zou XJ1,4; Greggain J1; Rodkaer SV5; Faergeman NJ5; Liang B[*]1,2; Watts JL[*]1 |
| 刊名 | JOURNAL OF LIPID RESEARCH
 |
| 出版日期 | 2013 |
| 卷号 | 54期号:9页码:2504-2514 |
| 关键词 | Caenorahbditis elegans fatty acid oxidation fatty acid synthesis oleic acid phosphatidylcholine phosphatidylethanolamine |
| 通讯作者 | liangb@mail.kiz.ac.cn ; jwatts@wsu.edu |
| 合作状况 | 其它 |
| 英文摘要 | Fatty acid desaturation regulates membrane function and fat storage in animals. To determine the contribution of stearoyl-CoA desaturase (SCD) activity on fat storage and development in the nematode Caenorhabditis elegans, we analyzed the lipid composition and lipid droplet size in the fat-6;fat-7 desaturase mutants independently and in combination with mutants disrupted in conserved lipid metabolic pathways. C. elegans with impaired SCD activity displayed both reduced fat stores and decreased lipid droplet size. Mutants in the daf-2 (insulin-like growth factor receptor), rsks-1 (homolog of p70S6kinase, an effector of the target of rapamycin signaling pathway), and daf-7 (transforming growth factor beta) displayed high fat stores, the opposite of the low fat observed in the fat-6;fat-7 desaturase mutants. The metabolic mutants in combination with fat-6;fat-7 displayed low fat stores, with the exception of the daf-2;fat-6;fat-7 triple mutants, which had increased de novo fatty acid synthesis and wild-type levels of fat stores. Notably, SCD activity is required for the formation of large-sized lipid droplets in all mutant backgrounds, as well as for normal ratios of phosphatidylcholine (PC) to phosphatidylethanolamine (PE). These studies reveal previously uncharacterized roles for SCD in the regulation of lipid droplet size and membrane phospholipid composition. |
| 收录类别 | SCI |
| 资助信息 | Support for this work was provided by National Institutes of Health Grant R01 DK-74114 (to J.L.W.) and National Natural Science Foundation of China Grants 31171134 (to B.L.) and 31160216 (to X.Z.). Some C. elegans strains were provided by the Caenorhabditis Genetics Center , which is funded by Offi ce of Research Infrastructure Programs, National Institutes of Health Grant P40 OD- 010440. |
| 语种 | 英语 |
| WOS记录号 | WOS:000330533600020 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7676]  |
| 专题 | 昆明动物研究所_脂类代谢与疾病 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.School of Life Science, University of Science and Technology of China, Hefei 230036, China 4.Department of Life Sciences and Biotechnology, Kunming University, Kunming, Yunnan 650214, China 5.Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark |
| 推荐引用方式 GB/T 7714 | Shi X,Li J,Zou XJ,et al. Regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase[J]. JOURNAL OF LIPID RESEARCH,2013,54(9):2504-2514. |
| APA | Shi X.,Li J.,Zou XJ.,Greggain J.,Rodkaer SV.,...&Watts JL[*].(2013).Regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase.JOURNAL OF LIPID RESEARCH,54(9),2504-2514. |
| MLA | Shi X,et al."Regulation of lipid droplet size and phospholipid composition by stearoyl-CoA desaturase".JOURNAL OF LIPID RESEARCH 54.9(2013):2504-2514. |
入库方式： OAI收割
来源：昆明动物研究所
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