The cytochrome b5 reductase HPO-19 is required for biosynthesis of polyunsaturated fatty acids in Caenorhabditis elegans
文献类型：期刊论文
| 作者 | Zhang YR1,3; Wang HZ1,6; Wu XY4; Su X5; Li TT5; Zou XJ[*]2; Liang B[*]1; Zhang JJ1,6; Hu Y1; Zhang LQ1,6 |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
 |
| 出版日期 | 2016 |
| 卷号 | 1861期号:4页码:310-319 |
| 关键词 | Cytochrome b5 reductase HPO-19 and T05H4.4 |
| 通讯作者 | xiaojuzou@163.com ; liangb@mail.kiz.ac.cn |
| 英文摘要 | Polyunsaturated fatty acids (PUFAs) are fatty acids with backbones containing more than one double bond, which are introduced by a series of desaturases that insert double bonds at specific carbon atoms in the fatty acid chain. It has been established that desaturases need flavoprotein-NADH-dependent cytochrome b5 reductase (simplified as cytochrome b5 reductase) and cytochrome b5 to pass through electrons for activation. However, it has remained unclear how this multi-enzyme system works for distinct desaturases. The model organism Caenorhabditis elegans contains seven desaturases (FAT-1, -2, -3, -4, -5, -6, -7) for the biosynthesis of PUFAS, providing an excellent model in which to characterize different desaturation reactions. Here, we show that RNAi inactivation of predicted cytochrome b5 reductases hpo-19 and T05H4.4 led to increased levels of C18:1n-9 but decreased levels of PUFA5, small lipid droplets, decreased fat accumulation, reduced brood size and impaired development. Dietary supplementation with different fatty acids showed that HPO-19 and T05H4.4 likely affect the activity of FAT-1, FAT -2, FAT -3, and FAT -4 desaturases, suggesting that these four desaturases use the same cytochrome b5 reductase to function. Collectively, these findings indicate that cytochrome b5 reductase HPO-19/T05H4.4 is required for desaturation to biosynthesize PUFAs in C. elegans. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13030600), National Natural Science Foundation of China (31401014, 31160216, 31171134, 31460268, U1202223 and U1402225), Yunnan Natural Science Foundation (2011FZ179, 2013FA042 and 2014FB180, and 2015FB177), Yunnan Provincial Sci- ence and Technology Department (2014HB022), Yunnan Oversea High-level Talents Program (2015HA040 to B L), and the West Light Foundation of the Chinese Academy of Sciences to YR Z. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10311]  |
| 专题 | 昆明动物研究所_脂类代谢与疾病 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key LaboratoryofAnimalModels and HumanDiseaseMechanismsofthe ChineseAcademy ofSciences, & YunnanProvince,KunmingInstituteofZoology, ChineseAcademy ofSciences, Kunming 650223, China 2.Department of Life Science and Biotechnology, Key Laboratory of Special Biological Resource Development and Utilization of University in Yunnan Province, Kunming University, Kunming 650214, China 3.College of Fisheries, Henan Normal University, Xinxiang, Henan 453007, China 4.Key Laboratory of Puer Tea Science, Ministry of Education (Yunnan Agricultural University), Kunming 650201, China 5.Department of Biochemistry and Molecular Biology, Soochow University Medical College, Suzhou 215006, China 6.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China |
| 推荐引用方式 GB/T 7714 | Zhang YR,Wang HZ,Wu XY,et al. The cytochrome b5 reductase HPO-19 is required for biosynthesis of polyunsaturated fatty acids in Caenorhabditis elegans[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,2016,1861(4):310-319. |
| APA | Zhang YR.,Wang HZ.,Wu XY.,Su X.,Li TT.,...&Zhang LQ.(2016).The cytochrome b5 reductase HPO-19 is required for biosynthesis of polyunsaturated fatty acids in Caenorhabditis elegans.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,1861(4),310-319. |
| MLA | Zhang YR,et al."The cytochrome b5 reductase HPO-19 is required for biosynthesis of polyunsaturated fatty acids in Caenorhabditis elegans".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 1861.4(2016):310-319. |
入库方式： OAI收割
来源：昆明动物研究所
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