Generation and characterization of a breast carcinoma model by PyMT overexpression in mammary epithelial cells of tree shrew, an animal close to primates in evolution
文献类型：期刊论文
| 作者 | Ge GZ1,2; Xia HJ1; Liu WJ1,2; Shao M1,2; Wang CY1; Xiao J1,2; Ge F1,5; Li FB1; He BL3; Li Y6 |
| 刊名 | INTERNATIONAL JOURNAL OF CANCER
 |
| 出版日期 | 2016 |
| 卷号 | 138期号:3页码:642-651 |
| 关键词 | PyMT animal model breast cancer tree shrew |
| 通讯作者 | chenc@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The tree shrew is becoming an attractive experimental animal model for human breast cancer owing to a closer relationship to primates/humans than rodents. Tree shrews are superior to classical primates because tree shrew are easier to manipulate, maintain and propagate. It is required to establish a high-efficiency tree shrew breast cancer model for etiological research and drug assessment. Our previous studies suggest that 7,12-dimethylbenz(a)anthracene (DMBA) and medroxyprogesterone acetate (MPA) induce breast tumors in tree shrews with a low frequency (<50%) and long latency (∼7-month), making these methods less than ideal. We induced mammary tumors in tree shrew (Tupaia belangeri chinensis) by injection of lentivirus expressing the PyMT oncogene into mammary ducts of 22 animals. Most tree shrews developed mammary tumors with a latency of about three weeks, and by 7 weeks all injected tree shrews had developed mammary tumors. Among these, papillary carcinoma is the predominant tumor type. One case showed lymph node and lung metastasis. Interestingly, the expression levels of phosphorylated AKT, ERK and STAT3 were elevated in 41-68% of PyMT-induced mammary tumors, but not all tumors. Finally, we observed that the growth of PyMT-induced tree shrew mammary tumors was significantly inhibited by Cisplatin and Epidoxorubicin. PyMT-induced tree shrew mammary tumor model may be suitable for further breast cancer research and drug development, due to its high efficiency and short latency. |
| 收录类别 | SCI |
| 资助信息 | Grant sponsor: Ministry of Science and Technology of China (863 projects); Grant numbers: 2012AA021801 and 2012AA022402; Grant sponsor: National Natural Science Foundation of China; Grant numbers: 81202110, 81120108019, U1132605 and 81325016; Grant sponsor: Chinese Academy of Sciences; Grant number: KSCX2-EW-J-23 |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9365]  |
| 专题 | 昆明动物研究所_肿瘤生物学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China 3.Department of Laboratory Animal Science, Kunming Medical University, Kunming, Yunnan, 650500, China 4.Department of Pathology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China 5.Department of Endocrine Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China 6.Lester and Sue Smith Breast Center, Baylor College of Medicine, Houston, Texas |
| 推荐引用方式 GB/T 7714 | Ge GZ,Xia HJ,Liu WJ,et al. Generation and characterization of a breast carcinoma model by PyMT overexpression in mammary epithelial cells of tree shrew, an animal close to primates in evolution[J]. INTERNATIONAL JOURNAL OF CANCER,2016,138(3):642-651. |
| APA | Ge GZ.,Xia HJ.,Liu WJ.,Shao M.,Wang CY.,...&Liu WJ.(2016).Generation and characterization of a breast carcinoma model by PyMT overexpression in mammary epithelial cells of tree shrew, an animal close to primates in evolution.INTERNATIONAL JOURNAL OF CANCER,138(3),642-651. |
| MLA | Ge GZ,et al."Generation and characterization of a breast carcinoma model by PyMT overexpression in mammary epithelial cells of tree shrew, an animal close to primates in evolution".INTERNATIONAL JOURNAL OF CANCER 138.3(2016):642-651. |
入库方式： OAI收割
来源：昆明动物研究所
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