The role of semaphorin 4D in tumor development and angiogenesis in human breast cancer
文献类型：期刊论文
| 作者 | Jiang HC1,2; Chen CS3; Gao CE5; Qiu LJ4; Liu WQ5; Yang J5; Jun N2; Dong J[*]2; Sun QM4; Wu J3 |
| 刊名 | ONCOTARGETS AND THERAPY
 |
| 出版日期 | 2016 |
| 卷号 | 9期号:X页码:5737-5750. |
| 关键词 | angiogenesis apoptosis breast cancer proliferation semaphorin 4D |
| 通讯作者 | jiandong18@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | Background: Semaphorin 4D (Sema4D) is highly expressed in certain types of tumors and functions in the regulation of tumor angiogenesis and growth. However, it is still not clear regarding the roles of Sema4D in breast cancer. This study was designed to explore the effects of Sema4D on proliferation, cell cycle progression, apoptosis, invasion, migration, tumor growth, and angiogenesis in breast cancer. Materials and methods: The expression level of Sema4D was investigated in MCF10A, 184A1, HCC1937, MDA-MB-468, MDA-MB-231, Hs578T, BT474, MCF-7, and T47D breast cancer cell lines by Western blotting analysis. Sema4D downregulation or overexpression was established by infection with lentiviruses-encoding Sema4D short hairpin RNA (shRNA) or Sema4D. To evaluate the effects of Sema4D on cell proliferation, cell cycle progression, apoptosis, invasion, and migration of MDA-MB-231 and MDA-MB-468 cells, methods including MTT assay, flow cytometry, wound healing assay, and transwell experiments were applied. BALB/c nude mice were injected with MDA-MB-231 cells, which were respectively infected with lentiviruses-encoding Sema4D, Sema4D shRNA, and GFP, followed by tumor angiogenesis assay. Results: Sema4D was expressed at higher levels in breast cancer cell lines compared with the normal human breast epithelial cell lines, especially in MDA-MB-231 and MDA-MB-468 cells. Cell proliferation ability was remarkably inhibited in Sema4D downregulated condition, whereas the proportions of cells in the G0/G1 phase and apoptosis increased in MDA-MB-231 and MDA-MB-468 cells. In addition, the invasion and migration abilities of these cells were obviously reduced. Xenograft growth as well as angiogenesis was inhibited when infected with lentiviruses-encoding Sema4D shRNA in vivo. Conclusion: Downregulation of Sema4D had notable influence on cell proliferation ability, invasion, migration, and apoptosis of both MDA-MB-231 and MDA-MB-468 cells. Furthermore, infection with lentiviruses-encoding Sema4D shRNA obviously inhibited tumor growth and angiogenesis in BALB/c nude mice. Our results showed that Sema4D may represent a novel therapeutic target for human breast cancer. |
| 资助信息 | This research was supported by the National Natural Science Foundation of China (grant number 81171946), the Natural Science Foundation of Yunnan Province (grant numbers 2011CA016 and 2012FB188), the Association Foundation Program of Yunnan Science and Technology Department and Kunming Medical University (grant number 2014FZ058), the Innovation Foundation of Kunming Medical University for PhD candidates (grant number 2014D07), the Training Project for Medical Science and Technology Experts in Kunming City (grant number SW-75), and the Foundation of Yunnan Medical Science and Technology (grant number 2016NS124). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10567]  |
| 专题 | 昆明动物研究所_肿瘤生物学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Department of Oncology, The affiliated children’s hospital of Kunming Medical University, 2.Department of Oncology, The Third affiliated hospital of Kunming Medical University, Yunnan Provincial Tumor hospital 3.Key laboratory of animal Models and human Disease Mechanisms of chinese academy of sciences and Yunnan Province, Kunming institute of Zoology, chinese academy of sciences, 4.Molecular epidemiology Joint laboratory, institute of Medical Biology, chinese academy of Medical sciences, Peking Union Medical college 5.Department of Oncology, The First affiliated hospital of Kunming Medical University, Kunming, People’s republic of china |
| 推荐引用方式 GB/T 7714 | Jiang HC,Chen CS,Gao CE,et al. The role of semaphorin 4D in tumor development and angiogenesis in human breast cancer[J]. ONCOTARGETS AND THERAPY,2016,9(X):5737-5750.. |
| APA | Jiang HC.,Chen CS.,Gao CE.,Qiu LJ.,Liu WQ.,...&Wu J.(2016).The role of semaphorin 4D in tumor development and angiogenesis in human breast cancer.ONCOTARGETS AND THERAPY,9(X),5737-5750.. |
| MLA | Jiang HC,et al."The role of semaphorin 4D in tumor development and angiogenesis in human breast cancer".ONCOTARGETS AND THERAPY 9.X(2016):5737-5750.. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。