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Fem1b promotes ubiquitylation and suppresses transcriptional activity of Gli1

文献类型：期刊论文

作者Gilder AS1; Chen YB1,2; Jackson RJ1; Jiang J2; Maher JF[*]1
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2013
卷号440期号:3页码:431-436
关键词Fem1b FEM-1 Gli1 TRA-1 Transcription Ubiquitin
通讯作者jmaher@umc.edu
合作状况其它
英文摘要The mammalian Fem1b gene encodes a homolog of FEM-1, a protein in the sex-determination pathway of the nematode Caenorhabditis elegans. Fem1b and FEM-1 proteins each contain a VHL-box motif that mediates their interaction with certain E3 ubiquitin ligase complexes. In C elegans, FEM-1 negatively regulates the transcription factor TEA-I, and functions as an E3 ubiquitin ligase substrate recognition subunit to target TRA-1 for ubiquitylation. TRA-1 is homologous to the mammalian Gli1 protein, a transcription factor that mediates Hedgehog signaling as well as having Hedgehog-independent functions. Whether the interaction between nematode FEM-1 and TRA-1 proteins is conserved, between corresponding mammalian homologs, has not been reported. Herein, we show that Fem1b interacts with Gli1 within cells, and directly binds GEL Fem1b also promotes ubiquitylation of Gli1, suppresses transcriptional activation by Gli1, and attenuates an oncogenic Gli1 autoregulatory loop in cancer cells, all dependent on the VHL-box of Fem1b. These findings have implications for understanding the cellular functions of Fem1b, and the regulation of Gli1 oncoprotein activity.
收录类别SCI
资助信息We thank the Molecular Core of the Center for Psychiatric Neuroscience, NIGMS – P20-RR-17701 (UMMC) for use of the lumi- nometer. We thank Joan W. Conaway (Stowers Institute for Medical Research) for the Flag-Fem1b L597A plasmid.
语种英语
WOS记录号WOS:000326857500013
公开日期2013-12-09
源URL[http://159.226.149.42:8088/handle/152453/7722]  
专题昆明动物研究所_肿瘤信号转导
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位1.Cancer Institute and Department of Medicine, University of Mississippi Medical Center, Jackson, MS 39216, United States
2.Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States
3.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan 650223, China
推荐引用方式
GB/T 7714		 Gilder AS,Chen YB,Jackson RJ,et al. Fem1b promotes ubiquitylation and suppresses transcriptional activity of Gli1[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,440(3):431-436.
APA Gilder AS,Chen YB,Jackson RJ,Jiang J,&Maher JF[*].(2013).Fem1b promotes ubiquitylation and suppresses transcriptional activity of Gli1.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,440(3),431-436.
MLA Gilder AS,et al."Fem1b promotes ubiquitylation and suppresses transcriptional activity of Gli1".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 440.3(2013):431-436.

入库方式： OAI收割

来源：昆明动物研究所

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