Meta-analysis of differentially expressed genes in osteosarcoma based on gene expression data
文献类型：期刊论文
| 作者 | Yang ZZ[*]1; Chen YB2; Fu Y3; Yang YH1; Zhang Y1; Chen YJ1; Li DQ1 |
| 刊名 | BMC MEDICAL GENETICS
 |
| 出版日期 | 2014 |
| 卷号 | 15期号:X页码:e80 |
| 关键词 | Meta-analysis Microarray Differentially expressed genes Osteosarcoma Expression data |
| 通讯作者 | yangzuozhangpre@163.com |
| 合作状况 | 其它 |
| 英文摘要 | Background: To uncover the genes involved in the development of osteosarcoma (OS), we performed a meta-analysis of OS microarray data to identify differentially expressed genes (DEGs) and biological functions associated with gene expression changes between OS and normal control (NC) tissues. Methods: We used publicly available GEO datasets of OS to perform a meta-analysis. We performed Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Protein-Protein interaction (PPI) networks analysis. Results: Eight GEO datasets, including 240 samples of OS and 35 samples of controls, were available for the meta-analysis. We identified 979 DEGs across the studies between OS and NC tissues (472 up-regulated and 507 down-regulated). We found GO terms for molecular functions significantly enriched in protein binding (GO: 0005515, P = 3.83E-60) and calcium ion binding (GO: 0005509, P = 3.79E-13), while for biological processes, the enriched GO terms were cell adhesion (GO:0007155, P = 2.26E-19) and negative regulation of apoptotic process (GO: 0043066, P = 3.24E-15), and for cellular component, the enriched GO terms were cytoplasm (GO:0005737, P = 9.18E-63) and extracellular region (GO: 0005576, P = 2.28E-47). The most significant pathway in our KEGG analysis was Focal adhesion (P = 5.70E-15). Furthermore, ECM-receptor interaction (P = 1.27E-13) and Cell cycle (P = 4.53E-11) are found to be highly enriched. PPI network analysis indicated that the significant hub proteins containing PTBP2 (Degree = 33), RGS4 (Degree = 15) and FXYD6 (Degree = 13). Conclusions: Our meta-analysis detected DEGs and biological functions associated with gene expression changes between OS and NC tissues, guiding further identification and treatment for OS. |
| 收录类别 | SCI |
| 资助信息 | This research was supported in part by grants (no. 81260322/H1606 and no.81372322/H1606) from the National Natural Science Foundation of China, a grant (no. 2012FB163) from the Natural Science Foundation of Yunnan Province, a grant (no. 2011FB201) from the Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University, and a grant (no. D-201242) from the specialty fund of high-level talents medical personnel training of Yunnan province. |
| 语种 | 英语 |
| WOS记录号 | WOS:000339401300001 |
| 公开日期 | 2014-10-10 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8032]  |
| 专题 | 昆明动物研究所_肿瘤信号转导 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Bone and Soft Tissue Tumors Research Center of Yunnan Province, Department of Orthopaedics, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming, Yunnan 650118, PR China 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China 3.Department of Dermatology, Beijing Hospital, Beijing 100730, PR China |
| 推荐引用方式 GB/T 7714 | Yang ZZ[*],Chen YB,Fu Y,et al. Meta-analysis of differentially expressed genes in osteosarcoma based on gene expression data[J]. BMC MEDICAL GENETICS,2014,15(X):e80. |
| APA | Yang ZZ[*].,Chen YB.,Fu Y.,Yang YH.,Zhang Y.,...&Li DQ.(2014).Meta-analysis of differentially expressed genes in osteosarcoma based on gene expression data.BMC MEDICAL GENETICS,15(X),e80. |
| MLA | Yang ZZ[*],et al."Meta-analysis of differentially expressed genes in osteosarcoma based on gene expression data".BMC MEDICAL GENETICS 15.X(2014):e80. |
入库方式： OAI收割
来源：昆明动物研究所
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