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  2. 昆明动物研究所
  3. 昆明动物研究所
  4. 动物模型与人类重大疾病机理重点实验室
  5. 重大疾病机理的遗传学
Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken

文献类型：期刊论文

作者Zhang ZR1,3; Zhu Q1; Yao YG2; Jiang XS3; Du HR4; Liu YP[*]1
刊名MOLECULAR BIOLOGY REPORTS
出版日期2012
卷号39期号:4页码:3517-3521
关键词Chicken CAPN3 qRT-PCR Ontogenic expression Tissue distribution
通讯作者liuyp578@yahoo.com
合作状况其它
英文摘要Calpain-3 is a skeletal muscle-specific protease and participates in the regulation of myogenesis. In this study, we quantified the expression of calpain-3 (CAPN3) mRNA in a Chinese local chicken breed (Sichuan Mountainous Black-boned chicken [MB]), to discern the tissue and ontogenic expression pattern. Meanwhile, we compared the CAPN3 mRNA expression pattern in MB chicken at 10 weeks with a commercial meat type chicken line (S01) of the same age to identify the unique expression pattern under different genetic background. A real time quantitative PCR (qRT-PCR) assay was developed for an accurate measurement of its expression in various tissues from chickens at different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Expression of the CAPN3 mRNA was detected in the selected tissues, regardless of age. The breast muscle and leg muscle tissues had a significantly higher expression than the other tissues from the same individual (P < 0.01). Overall, the CAPN3 mRNA level exhibited a "rise-decline" developmental change in detected tissues except for brain. The S01 chicken had a higher expression of the CAPN3 mRNA in detected tissues than the MB chicken at 10 weeks. The present expression data of chicken CAPN3 gene may provide some information to shed light on the tissue and ontogenic expression pattern during chicken development.
收录类别SCI
资助信息This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119).
语种英语
WOS记录号WOS:000301108500017
公开日期2012-04-05
源URL[http://159.226.149.42:8088/handle/152453/6900]  
专题昆明动物研究所_重大疾病机理的遗传学
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位1.College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Ya’an, Sichuan 625014, China
2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China
3.Sichuan Animal Science Academy, Chengdu, Sichuan 610066, China
4.Sichuan Dahen Poultry Breeding Company, Chengdu, Sichuan 610066, China
推荐引用方式
GB/T 7714		 Zhang ZR,Zhu Q,Yao YG,et al. Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken[J]. MOLECULAR BIOLOGY REPORTS,2012,39(4):3517-3521.
APA Zhang ZR,Zhu Q,Yao YG,Jiang XS,Du HR,&Liu YP[*].(2012).Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken.MOLECULAR BIOLOGY REPORTS,39(4),3517-3521.
MLA Zhang ZR,et al."Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken".MOLECULAR BIOLOGY REPORTS 39.4(2012):3517-3521.

入库方式： OAI收割

来源：昆明动物研究所

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