SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS
文献类型：期刊论文
| 作者 | Chen SY1; Yao YG2; Liu YP[*]1 |
| 刊名 | ANIMAL BIOTECHNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 23期号:3页码:213-220 |
| 关键词 | 12S rRNA gene Farm animals Mitochondrial DNA Restriction fragment length polymorphism Species identification |
| 通讯作者 | liuyp578@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | Bio-techniques such as genetic manipulation, marker-assisted selection, and identity test have largely facilitated the modern animal production practices. In the present study, we established a reliable and cost-effective molecular method of species identification for common farm animals. We first (re-)analyzed 179 mitochondrial 12S rRNA gene sequences of ten farm animal species to determine the intra-species and species-specific variations. The PCR-RFLP method was subsequently designed to identify these species by using endonucleases BshNI, ScaI, AluI, and BfaI. The poultry and livestock species were first discriminated by one double-digestion of both BshNI and ScaI, which generated different fragment patterns (325 bp and 115 bp for poultry vs. 364 bp and 76 bp for livestock). The ten species could be further discerned according to species-specific restriction pattern by subjecting to digestion of AluI and BfaI, respectively. Our approach would be more reliable by taking the intra-species variations into consideration and could be applied to species identity test, commercial fraud, and wildlife crime. |
| 收录类别 | SCI |
| 资助信息 | This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119). |
| 语种 | 英语 |
| WOS记录号 | WOS:000307353400007 |
| 公开日期 | 2012-09-11 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7065]  |
| 专题 | 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Chen SY,Yao YG,Liu YP[*]. SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS[J]. ANIMAL BIOTECHNOLOGY,2012,23(3):213-220. |
| APA | Chen SY,Yao YG,&Liu YP[*].(2012).SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS.ANIMAL BIOTECHNOLOGY,23(3),213-220. |
| MLA | Chen SY,et al."SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS".ANIMAL BIOTECHNOLOGY 23.3(2012):213-220. |
入库方式： OAI收割
来源：昆明动物研究所
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