Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)
文献类型：期刊论文
| 作者 | Liu XH1,4; Yao YG[*]1,2,3 |
| 刊名 | Zoological Research
 |
| 出版日期 | 2013 |
| 卷号 | 34期号:E2页码:E62−E68 |
| 关键词 | Chinese tree shrew Microsatellite Heterozygosity Individual identification Parentage testing |
| 通讯作者 | ygyaozh@gmail.com |
| 合作状况 | 其它 |
| 英文摘要 | The Chinese tree shrew (Tupaia belangeri chinensis) is a small experimental animal with a close affinity to primates. This species has long been proposed to be an alternative experimental animal to primates in biomedical research. Despite decades of study, there is no pure breed for this animal, and the overall genetic diversity of wild tree shrews remains largely unknown. In order to obtain a set of genetic markers for evaluating the genetic diversity of tree shrew wild populations and tracing the lineages in inbreeding populations, we developed 12 polymorphic microsatellite markers from the genomic DNA of the tree shrew. An analysis of a wild population of 117 individuals collected from the suburb of Kunming, China, showed that these loci exhibited a highly expected heterozygosity (0.616). These 12 microsatellites were sufficient for individual identification and parentage analysis. The microsatellite markers developed in this study will be of use in evaluating genetic diversity and lineage tracing for the tree shrew. |
| 收录类别 | 其他 |
| 资助信息 | This work was supported by the National 863 Project of China (2012AA021801) and grants from Chinese Academy of Sciences (KSCX2-EW-R-11 and KSCX2-EW-J-23) and Yunnan Province (2009CI119) |
| 公开日期 | 2013-06-14 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7475]  |
| 专题 | 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China 2.Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Tree Shrew Inbreeding Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.University of Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Liu XH,Yao YG[*]. Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)[J]. Zoological Research,2013,34(E2):E62−E68. |
| APA | Liu XH,&Yao YG[*].(2013).Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis).Zoological Research,34(E2),E62−E68. |
| MLA | Liu XH,et al."Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)".Zoological Research 34.E2(2013):E62−E68. |
入库方式： OAI收割
来源：昆明动物研究所
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